Oftalmol Zh. 2011; 4: 58-60.
http://doi.org/10.31288/oftalmolzh201145860
CLINICAL AND HISTOLOGICAL RESULTS OF EXPERIMENTAL USE OF IMPLANTS BASED ON CROSS-LINKED COLLAGEN AS DONOR CORNEA ANALOGUE
Pasechnikova N. V., Vit V. V., Leus M. F., Yakymenko S. A., Buznyk O. I.
Odessa, Ukraine
Bioengineered substitute of the corneal stroma (BSCS) was synthesized by porcine type I collagen cross-linking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide. The obtained transparent implant had optical and mechanical properties similar to the human cornea. It showed satisfactory biocompatibility when was implanted into rabbit corneas (8 rabbits — 8 eyes): BSCS has preserved its clarity during 12 months of the follow-up. Fine opacities of the superficial corneal layers have been observed in 2/8 eyes during the 1st postoperative week. In 1 eye opacity resolved, in the 2nd eye tiny fine opacity was formed. Light microscopy confirmed good integrity of the implants and absence of inflammation in the corneal stroma. Our data suggest that BSCS synthesized by collagen cross-linking might be a potential material for keratoplasty.
ЛИТЕРАТУРА
1. Применение сыворотки ожоговых реконвалесцентов при лечении ожогов глаз различной этиологии // дис. . . . канд. мед. наук, 14.00.08. — Одесса, 1968. — 421 с.
2.Пучковская Н. А., Якнменко С. А. Оптическое кератопротезирование. — К., «Здоров'я». — 1988. — 180 с.
3. Філатов В. П. Оптична пересадка рогівки і тканинна терапія. — К.: ДМВ, 1948. — 382 с.
4. Якименко С. А. 45 летние результаты кератопротезирования в Институте им. В. П. Филатова // Офтальмол. журн. — 2008. — № 3. — С. 13-18.
5. Якименко С. А., Бузник О. І. Трансплантація амніотичної оболонки в лікуванні важких опіків очей // Офтальмол. журн. — 2010. — № 3. — С. 47-51.
6. Dua H. S., Gomes J. A.P., King A. J., Maharajan V. S. Amniotic membrane in ophthalmology // Surv. Ophthal¬mol. — 2004. — Vol. 49. — P. 51-77.
Crossref
7.Fagerholm P., Lagali N. S., Carlsson D. J., Merrett K., Griffith M. Corneal regeneration following implantation of a biomimetic tissue-engineered substitute // Clin. Trans. Sci. — 2009. — Vol. 2. — P. 162- 164.
Crossref
8. Liu Y., Gan L., Carlsson D. J., Fagerholm P., Lagali N., et al. A simple, cross-linked collagen tissue substitute for corneal implantation // Invest. Ophthalmol. Vis. Sci. — 2006. — Vol. 47. — P. 1869- 1875.
Crossref
9. Merrett K., Fagerholm P., McLaughlin C. R., Dravida S., Lagali N. S., et al. Tissue engineered recombinant human collagen-based substitutes for implantation: performance of type I versus type III collagen // Invest. Ophthalmol. Vis. Sci. — 2008. — Vol. 49. — P. 3887-3894.
Crossref