https://doi.org/10.31288/oftalmolzh201467277

Influence of endotoxin-induced keratitis on the reducing potential of glutathione in the cornea of animals with experimental syndrome of the dry eye

Gaydamaka T. B. 1, Rafalyuk S. Ya. 2

1State Institution «The Filatov Institute of Eye Diseases and Tissue Therapy of National Medical Academy of Ukraine»; Odessa (Ukraine)

2Danylo Halytsky Lviv National Medical University; Lviv (Ukraine)

Introduction. Modern methods of treatment of keratitis are not always effective. Particular difficulties arise when treating keratitis in patients with symptoms of the dry eye.

Objective: To determine the degree of disturbances in the thiol system in the tissues of the anterior eye in the development of keratitis in animal models of dry eye syndrome.

Material and methods. In the experiment 36 Chinchilla rabbits weighing 2.2—2.9 kg were divided into 4groups: Group 1 — control (7rabbits), group 2 — under the experiment (6 rabbits), animals with the dry eye syndrome, Group 3 — experimental (12 rabbits), animals with keratitis, Group 4 — experimental (11 rabbits), animals with keratitis and dry eye syndrome.

Experimental keratitis was caused by intrastromal injection of 50 mcl of a 0.2 % solution of endotoxin — lipopolysaccharide phosphate buffer. For the development of the dry eye syndrome, there was used 0.1 % BAC solution prepared on the isotonic phosphate buffer (pH 7.3—7.4). Instillation was made daily (twice a day) for two weeks.

The concentration of reduced and oxidized glutathione in the cornea and lacrimal fluid was determinedspectrophotometrically by the methods of enzymatic analysis. The data obtained were subjected to statistical analysis using the package SPSS 11.0.

Results. The development of keratitis in animals with the dry eye syndrome is accompanied by a sharp reliable drop in reduced glutathione compared with keratitis without dry eye syndrome. Thus, on the 7th and 14th day of the observation the concentration of reduced glutathione in the cornea was reduced by 23.8 % and 21.8 % respectively.

The presence of the dry eye syndrome in the development of inflammation in the cornea causes a high degree of oxidative stress in it, as evidenced by the significant increase of the oxidized form of glutathione both in the cornea (21.8 %) — 7 days, 19.8 % — 14 hours), and tear (7 day — 26.3 % on day 14—46.0 %). 

Conclusion. Summarizing the data obtained in the study of the thiol status of the cornea in the development of inflammation in it in the conditions of the dry eye syndrome, it can be concluded that the sharp decline in the detoxification and reduction potential of the reduced/oxidized glutathione must be considered when determining the orientation of pathogenetic therapy of the pathological condition of the body.

Key words: keratitis, dry eye syndrome, a thiol system, experiment 

References

1.Anina EI. Prevalence of the cornea diseases among the population of Ukraine. Abstracts of II Black Sea International scientific conference of ophthalmologists. Odessa,2004:14.

2.Gaidamaka TB, Senishin VI, RAfalyuk SYa. Influence of the benzalkonium chloride eye drop preservative on the state of lysosomal membranes of the anterior eye tissues. Oftalmologiia. Vostochnaia Evropa. 2014;3(24): 86- 90. Russian.

3.Kamenskaia EV. The effectiveness of drug correction for the thiol status violations in the surface forms of herpetic keratitis: Author's thesis for Candidate of Med. Science: 14.01.10. Ophthalmology. Odessa; 2008. 20 p.

4.Naslyedov A. SPSS computer analysis of data in psychology and social sciences. Spb.: Piter; 2005. 416 p.

5.New methods of biochemical analysis. Izd. Leningradskogo univer; 1991. 395 p.

6.Petrunya AM, Mohamed Abdulrahman Kutayni. Petrunya AM, Mukhamed Abdulrakhman Kutaini. The study of metabolic processes in the cornea in experimental keratitis and conjunctivitis. Problemy ekologichnoi ta medichnoi genetiki I klinichnoi imunologii. 2012;3:45- 48. Russian.

7.Senishin VI, Rafalyuk SYa. Effect of the preservative benzalkonium chloride eye drops on the state of mitochon-drial enzymes tissues of the anterior eye. Oftalmol Zh. 2014;4:35-7. Russian.
Crossref

8.Barki WH, Tahir M. Effects of topical benzalkonium chloride on corneal epithelium. Biomedica. 2007;23:65- 70.

9.Bergmeyer HU. Methoden der enzymatischen Analyse. Herausgegeben von H. U. Bergmeyer. Berlin. 1986. 2198¬203.

10.Blanton CL, Rapuano CJ, Cohen EJ et al. Initial treatment of microbial keratitis. CLAO J. 1996;22(2):136- 10.

11.Bourcier T, Thomas F, Borderie V. Bacterial keratitis: pre-disposing factors, clinical and microbiological review of 300 cases. Br. J. Ophthalmol. 2003;87:834- 8.
Crossref

12.Hazlett LD. Role of innate and adaptive immunity in the pathogenesis of keratitis. Ocul. Immunol. Inflammat. 2005;13:133-8.
Crossref

13.Kim JR, Oh TH, Kim HS. Effect of benzalkonium chloride on the ocular surface of the rabbit. Jpn. J. Ophthalmol. 2011;55:283- 93.
Crossref

14.Limberg MB. A review of bacterial keratitis and bacterial conjunctivitis. Am J Ophthalmol. 1991;112 (4 Suppl):2S — 9S.

15.Lin Z, Liu X, Zhou T. A mouse dry eye model induced by topical administration of benzalkonium chloride. Mol. Vis. 2011;17:257- 64.

16.Norina T J, Raihan S, Bakiah S. Microbial keratitis: aetiological diagnosis and clinical features in patients admit¬ted to hospital universiti sains Malaysia. Singapore Med. J. 2008;49:67- 71.

17.Schultz CL, Morck DW, McKay SG. Lipopolysaccharide induced acute red eye and corneal ulcers. Exp. Eye Res. 1997;64:3-9.
Crossref

18.Schultz CL, Buret AG, Olson ME. Lipopolysaccharide entry in the damaged cornea and specific uptake by polymorphonuclear neutrophils. Infect. Immunity. 2000;68(3):1731- 4.
Crossref

19.Trocme S, Hwang L-J, Bean GW. The role of benzalkonium chloride in the occurrence of punctate keratitis: a meta-analysis of randomized, controlled clinical trials. Ann. Pharmacother. 2010;44:1914-21.
Crossref

20.Wilson SE, Netto M, Ambrosio R. Corneal cells: chatty in development, homeoatasis, wound healing, and disease. Am. J. Ophthalmol. 2003;136:530- 6.
Crossref

21.Xiong C, Chen D, Liu J. A rabbit dry eye model induced by topical medication of a preservative benzalkonium chlo¬ride. Invest. Ophthalmol. Vis. Sci. 2008;49:1850- 6.
Crossref

22.Yuan X, Wilhelmus KR, Matoba AY. Pathogenesis and outcome of paecilomyces Keratitis. Am. J. Ophthalmol. 2009;147:691- 6.
Crossref