https://doi.org/10.31288/oftalmolzh201643842

Study on the effect of quercetine and lipoate on lipid peroxidation in the retina in experimental diabetes

N.Pasyechnikova, Corr. Member of NAMS of Ukraine, Prof. 1

O.Moroz, Cand Sc (Med)2

1 SI "Filatov Institute of Eye Diseases and Tissue Therapy of NAMS of Ukraine "(Odessa, Ukraine), 

2 A. Nowak Transcarpathian Regional Clinical Hospital, Ophthalmology department (Uzhgorod, Ukraine)

E-mail: moroz.oleg@gmail.com

The experiment was performed on white rats and content of malondialdehyde and conjugated dienes in the retina and blood plasma in the condition of streptozotocine-induced diabetes development and quercetine and lipoate application was studied. In such conditions, the use of drugs studied significantly prevented an increase in temporary products of lipid peroxidation (LPO) in the retina and blood of streptozotocine-induced diabetic animals at all terms of diabetes development. The use of lipoic acid in a greater degree decreased the enrichment of final products of LPO in the retina and blood of streptozotocine-induced diabetic animals at all terms of diabetes development as compared to that when quercetine was used. 

Key words: streptozotocine-induced diabetes, retina, malondialdehyde, conjugated diene, experiment

 

References

1.Dedov II, Shestakova MV, Milenkaya TM. [Diabetes Mellitus: retinopathy, nephropathy]. M.: Meditsina; 2001. 175 p. In Russian.

2.Jain A, Sarraf D, Fong D. Preventing diabetic retinopathy through control of systemic factors. Curr. Opin. Ophthalmol. 2003;14(6):389-94.
Crossref   Pubmed

3.Veselovskaia ZF, Kindii TV. [Diagnostics of the preclinical stage and prediction of diabetic retinopathy progression]. Oftalmol Zh. 2001;1:13-6. In Ukrainean.

4.Pasechnikova NV, Naumenko VA, Zborovskaya AV. [Blood-retinal barrier state during diabetic retinopathy as per fluorometry data]. Oftalmol Zh. 2008; 5: 4-7. In Russian.

5.Poltorak VV, Blokh KO, Malashenko AM. [Experimental modeling of diabetes to study the specific effect of new antidiabetic agents]. Metodological recommendations. Kharkov; 1991. 19 p. In Russian.

6.Barber AJ. A new view of diabetic retinopathy: a neurodegenerative disease of the eye.  Prog. In Neuro-Psychopharm. & Biol. Psych. 2003;27:283 – 90.

7.Efimov A, Skorobonskaya N, Zuev N. [Diabetic neuropathy]. Liky Ukraine. 2005;(3):21–5. In Russian.

8.Leus NF. [Metabolic development mechanisms and pharmacotherapy aspects of diabetic retinopathy]. Oftalmol Zh. 2003; 5: 75-80. In Russian.

9.Forbes JM, Yee LTL, Thallas V. Advanced glycation end product interventions reduce diabetes-accelerated atherosclerosis. Diabetes. 2004;53:1813-23.

10.Kravchuk EA. [The role of free radical oxidation in pathogenesis of eye diseases]. Vestnik oftalmologii. 2004; 5: 48-51. In Russian.

11.Moroz OA. [Membrane-protective effect of quercetin and lipoate on the retina in streptozotocin diabetes]. Oftalmol Zh.2014;3:76-80. In Russian.

12.Bayness JW. Role of oxidative stress in development of complications in diabetes. Diabetes.  1991;40:405-12. 

13.Feillet-Coudry C, Rock E, Coudry C. Lipid peroxidation and antioxidant status in experimental diabetes. ClinChimActa. 1999;284:31 – 43.

14.Gutteridge JMC. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin. Chem. 1995;41:1819-28.

15.Kesavulu MM, Rao BK, Giri R. Lipid peroxidation and antioxidant enzyme status in Type 2 diabetic with coronary heart disease. Diabetes Res. Clin. Pract. 2001;53:33-9.

16.Reyk DM, Gillies MC, Davies MJ. The retina: oxidative stress and diabetes. Redox Rep. 2003;8 (4):187-92.

17.Stitt AW, Curtis TM. Advanced glycation and retinal pathology during diabetes.  Farmac. Rep. 2006;57:156 – 68.

18.Pavlyuchenko KP, Oleinik TV. [The sdudy of lipid perioxidation products in the development of streptozotocine-induced diabetes]. Vestnik neotlozhnoi I vosstanovitelnoi meditsiny. 2005; 6(3): 510-3. In Russian.  

19.Leus NF, Oleinik TV, Kolomiichuk SG. [Effect of vitamin B1 preparations (cocarboxylase and benfotiamine) on biophysical and metabolic processes in the retina and plasma albino rats with streptozotocin diabetes]. Oftalmol Zh. 2007;2:70–75. In Russian.

20.Mogilevskii SYu, Chuyko AL. [Influence of various vitamin В6 forms on the level of lipid perioxadation in the retina of the animal in streptozotocine diabetes]. Problemy ekologicheskoi I meditsinskoi genetiki I klinicheskoi immunologii. 2010;6(103):228-39. In Russian.

21.Andreeva LI, Kozhemiakin LA, Kishkun AA. [Modification of the method of determining lipid peroxidation in a test using thiobarbituric acid]. Lab Delo. 1988;(11):41-3. In Russian.  

22.Skornyakov VI, Kozhemyakin LA, Smirnov VV et al. [Lipid perioxidation products in cerebro-spinal fluid in patients with cerebrocranial trauma]. Lab. Delo. 1988;8:14-6. In Russian.  

23.Stal'naya ID Method of determination of conjugated diene of unsaturated higher fatty acid. Modern methods in biology, ed. VN Orekhovich. M.: Meditsina;1977. 63-4. In Russian.

24.Nasledov A. [SPSS computer data analysis in psychology and social sciences]. Spb.: Peter; 2005. 416 p. In Russian.