https://doi.org/10.31288/oftalmolzh201654753

Effect of PFCL versus heavy silicone oil on the structure and functional state of the rabbit retina

D.V. Zhmuryk,1 Cand Sc (Med)

V.V. Vit,2 Dr Sc (Med), Prof.

N.I. Molchaniuk,2 Cand Sc (Biol)

N.I. Khramenko,2 Cand Sc (Med)

M.V. Miliienko,1 MD

1 Kyiv Municipal Clinical Eye Hospital “Eye Microsurgery Center” 

2 Filatov Institute of Eye Diseases and Tissue Therapy, NAMS of Ukraine

Kyiv, Odessa, Ukraine

Background: The use of perfluorocarbon liquids (PFCL) and heavy silicone oil (HSO) for post-vitrectomy tamponade would potentially widen the range of indications for and improve outcomes of vitreoretinal surgery. The academic literature is, however, equivocal as to the effect of these substances on the structure and functional state of the retina.

Purpose: To ivestigate experimentally the effect of shor-term (14-day) tamponade with PFCL versus HSO on the structure and bioelectric functional activity of the rabbit retina over time.

Materials and Methods: A total of 6 Chinchilla rabbits (12 eyes) were used in the study. All animals were subjected to ERG analysis before posterior subtotal pars plana vitrectomy. The tamponade with PFCL and with HSO was performed in the right eye and left eye, respectively, and was removed after 14 days. Retinal structures were examined with light and electron miscroscopy and the second ERG was performed at day 7, day 14 or day 30 after removal of the tamponade.

Results: The analysis based on light and electron miscroscopy revealed similar effects of the two substances on the structures of the rabbit retina. Major changes were: (a) hydropic changes in the smooth endoplasmic reticulum of retinal pigment epithelium (RPE) cells, RPE mitochondria, photoreceptor inner segments, and ganglion and M?ller cells, and (b) compensation-and-restoration processes within these cells, which enable normalization of the structures. ERG recordings revealed no significant difference between the effect of PFCL and HSO on the functional activity of the rabbit retina: the amplitude of the response of the inner peripheral retina and that of the inner central retina were increased by 30.1% (supernormal ERG) and 44%, respectively, versus 43% and 64.7%, respectively.

Conclusions: Short-term (14-day) tamponades with PFCL and heavy silicone oil have similar effects on on the structure and functional activity of the rabbit retina, with the changes which are reversible and non-degenerative in nature. Restoration of bioelectric activity of the retina to baseline levels after removal of 14-day PFCL and heavy silicone oil tamponades requires a longer period than 30 days.

Key words: retina, ultrastructure, electroretinography, perfluorocarbon liquids, heavy silicone oil

 

References

1.Sirimaharaj M, Balachandran C, Chan WC, et al. Vitrectomy with short term postoperative tamponade using perfluorocarbon liquid for giant retinal tears. Br J Ophthalmol. 2005 Sep;89(9):1176-9
Crossref   Pubmed

2.Shkvorchenko DO, Levina LV. [On the tactics of surgical treatment of proliferative diabetic retinopathy complicated by anterior proliferative vitreoretinopathy]. Oftal’mokhirurgiya. 2006;1:29-32 Russian

3.Cillino S, Cillino G, Ferraro LL, Casuccio A .Treatment of persistently open macular holes with heavy silicone oil (Densiron 68) versus C2F6. A prospective randomized study. Retina. 2016 Apr;36(4):688-94
Crossref   Pubmed

4.Drury B, Bourke RD. Short-term intraocular tamponade with perfluorocarbon heavy liquid. Br J Ophthalmol. 2011 May;95(5):694-8
Crossref   Pubmed

5.Devin F, Jourdan T, Saracco JB, Lucciani A. [Experimental tolerability of perfluorodecalin in prolonged intraocular tamponade]. J Fr Ophthalmol.  1995;18(4):268-74 French

6.Chang S, Sparrow JR, Iwamoto T, et al. Experimental studies of tolerance to intravitreal perfluoro-n-octane liquid. Retina. 1991;11(4): 367-74
Crossref   Pubmed

7.Flores-Aguilar M, Munguia D, Loeb E, et al. Intraocular tolerance of perfluorooctylbromide (perflubron). Retina. 1995;15(1):3-13
Crossref   Pubmed

8.Mackiewicz J, Maaijwee K, L?ke C, et al. Effect of gravity in long-term vitreous tamponade: in vivo investigation using perfluorocarbon liquids and semi-fluorinated alkanes. Graefes Arch Clin Exp Ophthalmol. 2007 May;245(5):665-75

9.Bryan JS, Friedman SM, Mames RN, Margo CE. Experimental vitreous replacement with perfluorotri-n-propylamine. Arch Ophthalmol. 1994 Aug;112(8):1098-102

10.Killey FP, Edelhauser HF, Aaberg TA. Intraocular fluid dynamics: measurements following vitrectomy and intraocular sulfur hexafluoride. Arch Ophthalmol. 1980 Aug;98(8):1448-52

11.Winkler BS. The electroretinogram of the isolated rat retina. Vision Res. 1972 Jun;12(6):1183-98

12.Winkler BS, Simson V, Benner J. Importance of bicarbonate in retinal function. Invest Ophthalmol Vis Sci. 1977 Aug;16(8):766-8

13.Shkvorchenko DO. [Comprehensive surgical management of retinal detachments complicated by giant retinal dialysis and tears with the use of perfluorocarbon liquids] [Cand Sc (Med) Thesis]. Moscow: Fyodorov Eye Microsurgery Complex; 1995. 132 p. Russian