https://doi.org/10.31288/oftalmolzh201437680

Membrane-protective effect of quercetin and lipoate on the retina in streptozotocin diabetes

O. A. Moroz

Transcarpathian Regional Clinical Hospital. A. Novak, Uzhgorod (Ukraine)

Key words: experimental diabetes, the retina, lipoic acid, quercetin.

Introduction. Relevance of the work is to study the influence of quercetin and lipoate in treatment of experimental diabetes.

Purpose. To study the membrane — protective impact of quercetin and lipoate on the retina and in particular on the lysosomes of the pigment epithelium in streptezotocin diabetes.

Methods. Studies were conducted on white rats of the Wistar line weighing 180— 220 g. Two groups of animals with developing diabetes received oral lipoic acid and quercetin. Activity of lysosomal enzyme of the retina was determined using spectrophotometric assay methods.

Results. Application of the lipoic acid and quercetin has a pronounced effect on membrane-stabilizing effect on the retina at different periods of development of the diabetic process. Both studied drugs not only increased the stability of the intracel-lular membranes but also greatly prevented lysosomal degradation of the retinal structures.

Conclusions. The use of the lipoic acid and quercetin in development of experimental diabetes has the distinct stabilizing effect on the lysosomal membrane of the retina at different periods of the disease. The drugs under study largely inhibit the degradation of subcellular structures of the retina, which is a manifestation of some kind of the protective effect on the ultrastructural components of the visual analyzer in diabetes.

References

1.Alifanova TA, Kushnir NN. Diabetes and disability. Thesis of II Black Sea International conference. Odessa, 2004. 124.

2.Andrushkova OA, Antonyuk TN, Turuto TA. An integrated approach to the treatment of vascular changes in the eyes of patients with diabetes. Proceedings of I Int. Conf. «Modern aspects of vascular and endocrine diseases of the organ of vision» K., 2000. 85.

3.Gladush TI, Baidan EI. Stability of lysosomal membranes of the retina of albino rats with streptozotocin diabetes under medical exposure (acetylcysteine, flavonoids and taurine). Oftalmol Zh. 2010; 4:60-4. Russian.
Crossref

4.Evgrafov VYu. Diabetic retinopathy; pathogenesis, diagnostics, treatment: author's thesis for Doctor of Med. Science. M., 1996. 47 p.

5.Leus NF. Metabolic mechanisms of development and prospects of medical treatment of diabetic retinopathy. Oftalmol Zh. 2003;5:75-80. Russian.

6.Maltsev EV, Rodin SS, Chernyayeva SN. Diabetic retinopathy, mechanism of development. Oftalmol Zh. 2003;2:82-8. Russian.

7.Milenkaya TM, Besmertnaya EG. Diabetic retinopathy. Vrach. Delo. 2000;1:8-11. Russian.

8.Nasledov A. SPSS computer analysis of data in psychology and social sciences. SPb.:Piter; 2005. 416 p.

9.Nedzvetskaya OV. Modern trends in the treatment of diabetic retinopathy.  Mezhdunar.  Med. Zhurnal. 2000;3:56-8. Russian.

10.Pavlyuchenko KP, Oleinik TV. Oxidative damage to the retinal pigment epithelium in streptozotocin-induced diabetes modeling. Oftalmol Zh. 2005;3:47-9. Russian.

11.Sidorova MV. Diabetic retinopathy. Pathogenesis, clinic, treatment. K.:SMP «AVERS»; 2006. 156 p.

12.Aiello LP, Cahill MT, Wong JS. Systemic considerations in  the   management  of diabetic   retinopathy. Am. J. Ophthalmol. 2001;32:760-76.
Crossref

13.Bayness J. W. Role of oxidative stress in development of complications in diabetes. Diabetes. 1991;40:405-12.
Crossref

14.Bergmeyer HU. Methoden der enzymatischen Analyse. Herausgegeben von H. U. Bergmeyer. Berlin. 1986. 2254¬2265.

15.Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature. 2001;414:813-20.
Crossref

16.Dervan E, Lillis D, Flynn L. Factors that influence the patient uptake of diabetic retinoparthy screening. Irish. J. Med. Sci. 2008;177:303-8.
Crossref

17.Dierkx N, Horvath G, van Gils C. Oxidative stress status in patients with diabetes mellitus: relationship to diet. Eur J Clin Nutr. 2003;57(8):999-1008.
Crossref

18.Fong DS, Aiello LP, Ferris FL, Klein RK. Diabetic retinopathy. Ophthalmol. 2004;27:2540-53.
Crossref

19.Lang  G  E.   Pharmacological treatment of diabetic retinopathy. Ophthalmologica. 2007;221(2):112-7.
Crossref

20.Pan H-Z, Zhang H, Chang D et al. The change of oxidative stress products in diabetes mellitus and diabetic retinopathy. Br. J Ophthalmol. 2008;92:548-51.

21.Reyk DM, Gillies MC, Davies MJ. The retina: oxidative stress and diabetes. Redox Rep. 2003;8(4):187-92.

22.Zimmer P. Global and social implications of the diabetic epidemic. Nature. 2001;414:782-9.