https://doi.org/10.31288/oftalmolzh201755055

Morphological changes in the optic nerve after experimental injury followed by treatment with stem cells

I.V. Chepurnyi, A.V. Kopchak, A.V. Korsak, V.V. Likhodievskyi, O.I. Kovalchuk, S.S. Olefir, A.O. Zabila,  I.B. Chaikovskyi

Bogomolets National Medical University 

Kyiv (Ukraine)

E-mail:  yuchaika@i.ua                     

Background: Ocular trauma and its sequelae substantially affect quality of life. Effective methods to promote regeneration of damaged optic nerve (ON) are still to be developed.

Purpose: To investigate structural changes in the rat ON after experimental ON injury followed by treatment with stem cells.

Materials and Methods: After being subjected to experimental injury, 40 rats were divided into two equal groups depending on whether they did (Group II) or did not receive (Group I) stem cells derived from the bulge of hair follicles of syngeneic animals. Histological and neurohistological techniques were used to investigate structural changes in the traumatized rat ON.

Results: Experimental injury to the ON resulted in its degeneration. Stem cell grafting at the site of injury initiated improved ON regeneration, which was evidenced by (1) newly formed nerve fibers and glial columns mostly of oligodendrocytes, (2) reduced glial scar volume due to reduction in the number of astrocytes, and (3) rapid elimination of myelin debris.

Conclusion: Injection of neural crest stem cells derived from the bulge of hair follicles of syngeneic animals improves the optic nerve restoration after injury.

 

Key-words: optic nerve, trauma, regeneration, stem cells

References

1.Watanabe M. Regeneration of optic nerve fibers of adult mammals. Dev Growth Differ. 2010 Sep;52(7):567-76.
   Crossref   Pubmed

2.Wender M, Adamczewska-Goncerzewicz Z, Goncerzewicz A. Myelin lipids in Wallerian degeneration of the rabbit optic nerve. Exp Pathol (Jena). 1979;17(6):334-9
   Crossref   Pubmed

3.Gall C, Lucklum J, Sabel BA, Franke GH. Vision- and Health-Related Quality of Life in Patients with Visual Field Loss after Postchiasmatic Lesions. Invest Ophthalmol Vis Sci. 2009; 50:2765–76
   Crossref   Pubmed

4.Greenwald B, Kapoor N, Singh A. Visual impairments in the first year after traumatic brain injury. Brain Inj. 2012;26(11):1338-59
   Crossref   Pubmed

5.Fischer D, Leibinger M. Promoting optic nerve regeneration. Prog Retin Eye Res. 2012 Nov;31(6):688-701
   Crossref   Pubmed

6.Forbes SJ, Rosenthal N. Preparing the ground for tissue regeneration: from mechanism to therapy. Nat Med. 2014 Aug;20(8):857-69

7.Maclaren RE. Regeneration and transplantation of the optic nerve: developing a clinical strategy. Br J Ophthalmol. 1998 May;82(5):577-83.

8.Pernet V, Joly S, Dalkara D, et al. Long-distance axonal regeneration induced by CNTF gene transfer is impaired by axonal misguidance in the injured adult optic nerve. Neurobiol Dis. 2013 Mar;51:202-13.

9.Pernet V, Joly S, Jordi N, et al. Misguidance and modulation of axonal regeneration by Stat3 and Rho/ROCK signaling in the transparent optic nerve. Cell Death Dis. 2013 Jul 18;4:e734

10.Pernet V, Schwab M. Lost in the jungle: new hurdles for optic nerve axon regeneration. Trends Neurosci. 2014 Jul;37(7):381-7.

11.Vit VV. [The structure of the human visual system]. Odessa:Astroprint; 2003 Russian

12.Kordium V, Chaikovsky Yu, Irodov D, Drahulian M, et al. Modelling of systemic lesion of organism for development of multitarget cellular and cytokine therapy. Biopolym Cell. 2016;32(5):381-94.

13.Liu B, Hunter D, Smith A, et al. The capacity of neural crest-derived stem cells for ocular repair. Birth Defects Res C Embryo Today. 2014 Sep;102(3): 299-308. 

14.Vasyliev R., Rodnichenko A., Shamalo S. et al. Effects of Neural Crest-Derived Multipotent Stem Cells on Regeneration of an Injured Peripheral Nerve in Mice. Neurophysiology. 2015;47(1):80–83

15.Sieber-Blum M, Schnell L, Grim M, et al. Characterization of epidermal neural crest stem cell (EPI-NCSC) grafts in the lesioned spinal cord. Mol Cell Neurosci. 2006 May-Jun;32(1-2):67-81. 

16.Sieber-Blum M. Epidermal neural crest stem cells and their use in mouse models of spinal cord injury. Brain Res Bull. 2010 Oct 30;83(5):189-93

17.Biernaskie J, Sparling J, Liu J, et al. Skin-Derived Precursors Generate Myelinating Schwann Cells That Promote Remyelination and Functional Recovery after Contusion Spinal Cord Injury. J Neurosci. 2007 Sep 5;27(36):9545-59

18.Dupin E, Coelho-Aguiar J. Isolation and differentiation properties of neural crest stem cells. Cytometry A. 2013 Jan;83(1):38-47

19.Krejci E, Grim M. Isolation and characterization of neural crest stem cells from adult human hair follicles. Folia Biol (Praha). 2010;56(4):149-57

20.Sieber-Blum M, Grim M, Hu YF, Szeder V. Pluripotent neural crest stem cells in the adult hair follicle // Dev Dyn. 2004 Oct;231(2):258-69.

21.Sieber-Blum M, Grim M. The adult hair follicle: Cradle for pluripotent neural crest stem cells. Birth Defects Res C Embryo Today. 2004 Jun;72(2):162-72.

22.Yang R, Xu X. Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles. J Vis Exp. 2013 Apr 6;(74)

23.Kolomiitsev A, Chaikovsky Yu, Tereschenko T. [Rapid method for impregnation of the components of the peripheral nervous system with silver nitrate]. Morfologiia (form. Arkh Anat Gistol Embriol). 1981;8:93-6. Russian

24.Morgan-Warren PJ, Berry M, Ahmed Z, et al. Exploiting mTOR Signaling: A Novel Translatable Treatment Strategy for Traumatic Optic Neuropathy? Invest Ophthalmol Vis Sci. 2013 Oct 23;54(10):6903-16

25.Narciso M, Hoko? J, Martinez A. Watery and dark axons in Wallerian degeneration of the opossum's optic nerve: different patterns of cytoskeletal breakdown? An Acad Bras Cienc. 2001 Jun;73(2):231-43.

26.Saggu SK, Chotaliya HP, Blumbergs PC, Casson RJ. Wallerian-like axonal degeneration in the optic nerve after excitotoxic retinal insult: an ultrastructural study. BMC Neurosci. 2010;11:97

27.Yu F, Zhang R. A novel model of optic nerve injury established by microsurgery using the pterional approach in cats. Neurol India. 2011 May-Jun;59(3):355-61