https://doi.org/10.31288/oftalmolzh201761115

Investigation of optical density of aqueous humor samples from patients with combined cataract and POAG and those with cataract only with the use of spectroscopy

V.O. Mel’nik, Cand Sc (Med)

Visiobud+ Clinic LLC

Kyiv, Ukraine

Background: The importance of the subject is evidenced by the steadily increasing number of patients with primary-open angle glaucoma (POAG) and absence of a unified theory for the development of POAG.

Purpose: To compare the absorption spectra of the aqueous humor (AH) samples from patients with combined cataract and POAG, and those from patients with cataract only, and to identify differences, if any, in parameters of these spectra between the two groups.

Materials and Methods: AH samples were obtained from 96 patients (96 eyes including 36 eyes with combined cataract and POAG, and 60 eyes with cataract only), and their absorption spectra were investigated.

Results: In the AH samples obtained from patients with combined cataract and POAG or those with cataract only, two absorption peaks were found at 202 nm and 267 nm. The mean optical density at 202 nm of the AH samples obtained from the former patients was higher than that of the samples obtained from the latter patients. The intergroup difference in the optical density at 202 nm (but not at 267 nm) of the AH samples was statistically significant.

Conclusion: A statistically significant difference in the optical density of AH samples was found between patients with POAG and those without POAG based on the results of spectroscopy that can be regarded as a valuable adjunct in the diagnosis of patients with this disorder.

Keywords: primary-open angle glaucoma, cataract, spectroscopy, optical density, aqueous humor pressure   

References

1. Quigley HA, Broman AT. The number of people with glaucoma worldwide in 2010 and 2020. Br J Ophthalmol. 2006;90:262-67
   Crossref   Pubmed
2. Alekseev VN, Gazizova IR.  [Neurodegenerative changes in patients with primary open-angle glaucoma]. Prakticheskaya meditsina. 2012; 4:154-156. Russian
3. Alekseev VN, Malevannaya OA. [Quality of life in patients with POAG]. In: [Proceedings of the Russian National Conference on Issues and Solutions for Combating Glaucoma]. November 23-25, 2004, Moscow, Helmholtz Research Institute of Eye Diseases. pp. 389-393. Russian
4. Egorov EA, Stavitskaya TV, Nalobnova IuV. [Electro- and psychophysiological methods for early glaucoma diagnostics]. Klinicheskaya oftalmologiya. 2003;4(2):68-70. Russian
5. Kolotilov SV, Mel’nik VO. [Comparing spectroscopic characteristics of the intraocular humor from patients with cataract and patients with primary open-angle glaucoma]. Fiziolohichny? zhurnal. 2016;62(5):62-69. Ukrainian
6. Grus FH, Joachim SC, Pfeiffer N. Proteomics in ocular fluids. Proteomics Clin Appl. 2007 Aug;1(8):876-88.
   Crossref   Pubmed
7. Scholtzer-Schrehardt U, Lommatzsch J, Kuchle M, et al. Matrix metalloproteinases and their inhibitors in aqueous humor of patients with pseudoexfoliation syndrome/glaucoma and primary open-angle glaucoma. Invest Ophthalmol Vis Sci. 2003 Mar;44(3):1117-25.
   Crossref   Pubmed
8. Solovyeva NI. [Basic metalloproteinases of the connective-tissue matrix]. Bioorganicheskaya khimiya. 1994;20(2): 143-152. Russian
9. Hu DN, Ritch R, Liebmann J, et al. Vascular endothelial growth factor is increased in aqueous humor of glaucomatous eyes. J Glaucoma. 2002 Oct;11(5):406-10
   Crossref   Pubmed
10. Vesaluoma M, Mertaniemi P, Mannonen S, et al. Cellular and plasma fibronectin in the aqueous humor of primary open-angle glaucoma, exfoliative glaucoma and cataract patients. Eye. 1998;12886-890
    Crossref   Pubmed
11. Richer SP, Rose RC. Water soluble antioxidants in mammalian aqueous humor: interaction with UV B and hydrogen peroxide. Vis Res. 1998 Oct;38(19):2881-8.
    Crossref   Pubmed
12. De Berardinis E, Tieri O, Polzella A, Iuglio N. The chemical composition of the human aqueous humour in normal and pathological conditions. Exp Eye Res. 1965 Sep;4(3):179-86.
    Crossref   Pubmed
13. Prata TS, Navajas EV, Melo LA Jr, et al. [Aqueous humor protein concentration in patients with primary open-angle glaucoma under clinical treatment]. Arq Bras Oftalmol. 2007 Mar-Apr;70(2):217-20.
    Crossref   Pubmed
14. Sverdlova OV. [Electronic spectra in organic chemistry]. Leningrad: Khimiya, 1985. Russian
15. McLaughlin CW(1), Karl MO, Zellhuber-McMillan S. Electron probe X-ray microanalysis of intact pathway for human aqueous humor outflow. Am J Physiol Cell Physiol. 2008;295:C1083–1091
    Crossref   Pubmed
16. Al-Aswad LA, Gong H, Lee D, et al. Effects of Na-K-2Cl cotransport regulators on outflow facility in calf and human eyes in vitro. Invest Ophthalmol Vis Sci. 1999; 40: 1695–1701
17. Gabelt BT, Kaufman PL. Changes in aqueous humor dynamics with age and glaucoma. Prog Retin Eye Res. 2005 Sep;24(5):612-37
    Crossref   Pubmed