J.ophthalmol.(Ukraine).2022;3:39-44.
http://doi.org/10.31288/oftalmolzh202233944
Received: 27.01.2022; Accepted: 25.04.2022; Published on-line 15.06.2022
Influence of corvitin and metformin on biochemical changes in lacrimal glands of rats during water avoidance stress modeling
Ye. K. Matsytska, O. Ye. Akimov, A. O. Mykytenko
Poltava State Medical University; Poltava (Ukraine)
TO CITE THIS ARTICLE:Matsytska YeK, Akimov OYe, Mykytenko AO. Influence of corvitin and metformin on biochemical changes in lacrimal glands of rats during water avoidance stress modeling. J.ophthalmol.(Ukraine).2022;3:39-44. http://doi.org/10.31288/oftalmolzh202233944
Background. Dry eye disease is a multifactorial condition, which is characterized by impairment of tear film formation. Lacrimal glands metabolism plays a critical role in dry eye disease. Emotional stress may impair lacrimal glands function.
Purpose. We aimed to study production of nitric oxide from constitutive and inducible NO-synthases, activity of arginases and oxidative stress markers in lacrimal glands of rats during modeling of water avoidance stress (WAS) and its correction by metformin and corvitin.
Material and methods. We concluded our experiment on 36 adult male rats of Wistar line weighing 190-240 g. Animals were divided into 6 groups consisting from 6 animals each, namely: control group, WAS group, group of correction by metformin (200 mg/kg) and group of correction by corvitin (10 mg/kg) during WAS modeling. And two drug-control groups.
Results. WAS leads to increased activity of inducible NO-synthase, superoxide dismutase, catalase and concentration of MDA by 1.59, 1.93, 1.97 and 1.28 times respectively. Metformin and corvitin decreased activity of inducible NO-synthase by 8.25 and 8.5 times respectively, concentration of MDA decreased by 1.35 and 1.26 times respectively. Activities of superoxide dismutase did not change after introduction of metformin and corvitin. Metformin decreased catalase activity by 1.47 and corvitin increased it by 1.55 times. Production of superoxide dropped during WAS by 1.59 times and was increased to level below or equal that of control animals with introduction of metformin and corvitin.
Conclusion. Increased activity of inducible NO-synthase during WAS is a possible reason of tissue damage in lacrimal glands of rats. Introduction of metformin or corvitin during WAS are an effective means for correction of tissue damage in lacrimal glands of rats due to their ability to lower increased inducible NO-synthase activity.
Кey words: corvitin, metformin, lacrimal glands, water avoidance stress
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Information about authors and disclosure of information
Corresponding author: Mykytenko A.O., e-mail: mykytenkoandrej18@gmail.com.
Author contribution: Matsytska Ye.K.: Conception; Research; Visualization; Writing an initial project. Akimov O.Ye.: Research; Methodology; Formal analysis; Validation; Writing - reviewing and editing. Mykytenko A.O.: Conception; Overall responsibility; Validation; Writing - reviewing and editing. All authors analyzed the results and approved the final version of the manuscript.
Disclaimer: Authors state that the opinions expressed in the submitted article are their own, and do not reflect official positions of their institution
Financial support: there is no financial support for this work
COI statement: Authors certify that we have no actual or potential conflict of interest (financial, personal, professional, or other interests) that I believe may be relevant to the subject matter or materials described and discussed in this manuscript.
Abbreviations: WAS – water avoidance stress; ROS – reactive oxygen species; Nfr-2 – erythroid-2–related factor 2; SOD – superoxide dysmutases; CAT – catalase; NF-κB – nuclear factor kappa-light-chain-en hancer of activated B cells; iNOS – induc ible NO-synthase; MDA – malondialdehyde; NO2- – nitrites; ONOO- – peroxinitrites; LPO – lipid peroxidation; NO – nitric oxide; O2 – oxygen; O2•- – superoxide anion radical; CO2 – carbon dioxide; carbon trioxide (CO3•-); nitrate anion (NO3-).