https://doi.org/10.31288/oftalmolzh201351923

The level of tumor necrosis factor a and vascular endothelial growth factor in patients with rhegmatogenous retinal detachment with proliferative vitreoretinopathy of varying degrees

Levitskaya GV

SI «The Filatov Institute of Eye Diseases and Tissue Therapy NAMS of Ukraine», Odessa

E-mail: kid_od@mail.ru

Background. It is known that growth factors, including tumor necrosis factor a (TNF-a) and vascular endothelial growth factor (VEGF) performs regulation of intercellular and intersystem interactions, also they possess the ability to regulate the proliferative processes. Therefore, the study of changes in cytokine profile in patients with rhegmatogenous retinal detachment (RRD) is necessary to gain a better understanding of the pathogenesis of RRD and to develop new methods of treatment. 

Purpose. To detect expression patterns of tumour necrosis factor a (TNF-a) and vascular endothelial growth factor (VEGF) in the vitreous and vitreous fluid of rhegmatogenous retinal detachment (RRD) patients with varying degrees of proliferative vitreoretinopathy (PVR).

Material and methods. Standard ophthalmic examination was performed in 79 RRD patients. Patients were divided into three groups — PVR A, PVR B and PVR C. In the vitreous and vitreous contents TNF-a and VEGF level was evaluated by ELISA.

Results. In cases with PVR A tumor necrosis factor a level in vitreous was 39.98+21.95 pg/ml and increased as increasing proliferation — 2 and 3.5 times in grades B and C. In vitreal contents it increased in PVR B — 1.4 times and PVR C — 2.5 times. Concentration of VEGF in vitreous was also increasing by enhancing PVR: grade A to B — 1.7times, PVR grade A to C— 2 times. In vitreal contents VEGF level in PVR grade B group was higher by 31.7 % and in PVR grade C by 150 % than in PVR grade A group. A direct strong correlation was found between cytokines level in the vitreous and vitreous contents and degree of PVR. 

Conclusion. In vitreous and vitreous humor of RRD patients cytokine profile changes were detected in terms of increasing VEGF and TNF-a concentrations depending on the PVR stage.

Key words: rhegmatogenous retinal detachment, proliferative vitreoretinopathy, tumor necrosis factor a, vascular endothelial growth factor, vitreous, vitreous contents

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