https://doi.org/10.31288/oftalmolzh201357882

A content of ATP and glucose-6-phosphate in modeling alkaline burn and possibilities of its correction by cytoflavin

Chalanova RI, Kolomiychuk SG, Baidan EI, Mbarki M

SI «Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine, Odessa, Ukraine

Relevance. Taking into account significance of energy supply of the reparative regeneration, it is important to determine the state of the energy potential of the cornea according to the data of the content of phosphor energy compounds (ATP and glucose-6-phosphate) in dynamics of the burning process and possibility of its restoration in treatment with intravenous infusions of cytoflavin. 

Purpose. To study the state of bioenergetics processes in the cornea in modeling alkaline burn of the eyes and possibilities of its correction by cytoflavin. 

Material and methods. The study was conducted in two groups of rabbits with modelled alkaline burn of both eyes, which had been instilled disinfectant drops and tobrex from the moment of injury. In the main group the treatment was added by 5 intravenous infusions of cytoflavin from 10 to 14 day from the moment of burn. On the 3rd, 7th, 21st, 30th and 45th day of burn the animals were taken out of the experiment in the state of ethaminal — sodium anesthesia for taking corneas for biochemical studies of the content of ATP and glucose-6-phosphate. 

Results. There was established twice reduction of ATP and glucose-6-phosphate during seven days from the moment of burn. The content of these compounds reliably decreased considerably under the influence of cytoflavin in the main group compared with the controls from the 21st to 45th day.

Key words: alkaline burn of the cornea, regeneration, energy supply, cytoflavin, experiment

References

1.Andrushkova OA, Bulgakova TYu. On a search of new ways of treatment of eye burns. Scientific conference of oph-thalmologists, dedicated to 90th anniversary of N. Puch-kovskaya. Proceedings. Odessa; 1998.

2.Volkov VV. On parts of pathogenesis of eye burns. Simposium in treatment ofeye burns. Proceedings. M.; 1989. 3- 4.

3.Gundorova RA. Systemization of clinic, diagnosis and treatment of corneal lesions in trauma disease of the vision organ. Fedorov Memorial Lectures — 2004. New technologies in the treatment of corneal diseases: Russian scient. pract. conference with international participation. Proceedings. 2004; Moscow. 94-9.

4.Nasledov A. SPSS computer analysis of the data in psy-chology and social sciences. StP.: Piter; 2005. 416 p.

5.New methods of biochemical analysis. Izd. Leningradsk-ogo univer. 1991. 395 p.

6.Yakimenko SA, Panko OM. Application of Erbisol in treat-ment of chemical eye burns. Oftalmol Zh. 2003; 1: 26-9.

7.Bergmeyer HU. Methoden der enzymatischen Analyse. He-rausgegeben von H. U. Bergmeyer. Berlin; 1986. 2198- 203.

8.Falder S, Silla R, Phillips M. Thiamine supplementation increases serum thiamine and reduces pyruvate and lactate levels in burn patients. Burns. 2010; 36: 261- 9.
Crossref

9.Herboth T, Geerling G, Duncker G. Consequent use of the Aachen therapy concept after severe chemical burns of both eyes. Klin. Monatsbl. Augenheilkd.1998; 212: 166-9.
Crossref

10.Kuckelkorn R, Schrage N, Keller G. Emergency treatment of chemical and thermal eye burns. Acta Ophthalmol. Scand. 2002; 80: 4-10.
Crossref

11.Mokline A, Charsallah L, Abdenneji A. Lactate in burn patients: biomarker of sepsis and mortality. Critical Care; 2012; 16: 258.
Crossref

12.Onofrey BE. Management of corneal burns. Optom Clin. 1995; 4: 31-40.

13.Schrage NF, Langefeld S, Zschocke J. Eye burns: an emergency and continuing problem. Burns. 2000; 26: 689-99.
Crossref

14.Seong MC, Kim MJ, Tchah HW. Chemical burns of the cornea due to soda lime used for line marking on the play-ground. J. Korean Ophthalmol. Soc. 2005; 46: 1737-40.

15.Shirzadeh E. Bilateral Chemical Burns of the Cornea Due to Limewater: A Specific case. Iran. Red Cresc. Med. J. 2013; 15: 11-2.
Crossref

16.Slobodin VB. Metabolic activity of Krebs cycle in the liver during experimental burns. Vorp. Med. Khim. 1982; 28: 93-6.