https://doi.org/10.31288/oftalmolzh201452023

Allelic variant frequency of the promotor CRYG gene in patients with initial and immature cataract

S. A. Rykov1, Y Y Byts1, S. V. Goncharov2, V. E. Dosenko2

1 «Eye Microsurgery Center»,

2 «Bogomoletz Institute of Physiology»;

Kiev (Ukraine) 

Key words: crystallin, single nucleotide polymorphism, cataract.

Purpose. To investigate the genetical precursors of cataract development the next groups were included: patients sufferingfrom cataract (96) and 96 healthy persons. Method. The determination of gamma-crystallin polymorphism (G-47—A) (rs2289917) was provided using PCR method and further analyses of restriction fragment length polymorphism.

Results. These allelic variants have the significant different: G/G — 35,37 %, G/A — 53,66 %, A/A — 10,98 %,, and G/G — 55,06 %, G/A — 35,96 %,, A/A — 8,99 % comparing with the control group (p=0,03, by %2-test). Allelic frequency determination also gives us statistically significant differences between cataract patients and control — minor allele frequency 0,38 and 0,27 correspondingly, p=0,03.

Conclusion. Thus data received show that polymorphism of gamma-crystallin gene is associated with the cataract development in Ukrainian population.