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http://doi.org/10.31288/oftalmolzh201247884

ANTIPROLIFERATIVE ACTION OF RANIBIZUMAB, BEVACIZUMAB AND PEGAPTANIB ON FIBROBLAST-LIKE CELL STRAIN IN VITRO

A. Sergiienko, L. Lytvynchuk, G. Lavrenchuk

Kyiv, Ukraine

The efficacy of intravitreal injections of anti-VEGF in the case of choroidal neovascularization (CNV) due to pathologic myopia becomes apparent in particular with decreasing of membrane's dimensions. The authors have investigated antiproliferative and apoptotic action of anti-VEGF agents (ranibizumab, bevacizumab, pegaptanib) on fibroblast-like cell strain L929 in vitro (testing model of CNV cellular matrix in vitro). Cultivation of investigated drugs with cellular culture was held according to standard protocol. During investigation cellular vital indices were evaluated (survival, mitotic activity, polycariocyte index). Analysis of cellular apoptosis level was evaluated as well. Cultivation of cellular culture L929 with anti-VEGF agents has provoked depression of cellular vital indices that was manifested with decrease of survival, mitotic index and increase of polycariocytes, which are signs of cellular reproductive death. Apoptosis has increased as a result of action of all drugs. Antiproliferative and apoptotic effects were dependent on drug's doses. Obtained results reveal and approve an alternative actions of anti-VEGF drugs on fibroblast-like cells: depression of mitotic activity and apoptosis increase.

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