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http://doi.org/10.31288/oftalmolzh201144448

THE ROLE OF THE VITREOUS HUMOR AND SUBRETINAL FLUID IN PROLIFERATION PROCESSES IN RETINAL DETACHMENT

A. Sergienko, G. Lavrinchuk, O. Vlasko, L. Lytvynchuk

The aim was to evaluate general content effects of vitreous and subretinal fluid on fibroblast-like cell strain, as model of proliferative processes in vitro, and to find out the evidences that proliferative vitreoretinopathy (PVR) de¬velopment is dependant not only on retinal pigment epithelium migration. For that reason 10 vitreous body aspirates and 9 subretinal fluid samples, taken during PPV in patients with retinal detachment, were added to cell strain L929 which obtains an ability to proliferate permanently. Cellular effects were evaluated according to cellular vital activity indices changes: survival, mitotic index and polycaryocyte index. Vitreous aspirates have induced cellular adhesion decrease and increase of mitotic activity. Subretinal fluid samples have reduced cellular reproducibility of the cell strain. Data analysis revealed the ability of subretinal fluid content to depress cellular reproducibility. On the contrary vitreous content revealed the ability to stimulate cellular mitosis and proliferation. Given results suggest that there is a favorable environment existence in vitreous cavity for PVR development.

ЛИТЕРАТУРА

1.Запускалов И. В., Кривошеина О. И. О патогенезе пролиферативной витреоретинопатии // Современные технологи лечения витреоретинальной патологи. — М.: МНТК им. С. Н. Федорова, 2002. — С. 110-115.

2.Путиенко А. А. Анализ результатов лечения регматогенной отслойки сетчатки, осложненной передней пролиферативной витреоретинопатией // Офталь-мол. журн. — 2003. — № 1. — С. 8-11.

3.Зуева, И. В. Цапенко, М. В. Рябина и др. Роль клеток Мюллера в патогенезе пролиферативных изменений сетчатки // Материалы III Междунар. науч. — практ. конф. «Пролиферативный синдром в офтальмологии». — М., 2004 г. — С.27.

4.Cook J. B., Lewis G. P., Fisher S. K. et al. Apoptotic pho-toreceptor degeneration in experimental retinal detach¬ment // Invest. Ophthalmol Vis Sci. — 1995. — V.36. — P.990-996.

5.Hisatomi T., Sakamoto Т., Murata T. et al. Relocation of apoptosis inducing factor in photoreceptor apoptosis induced by retinal detachment in vivo // Am. J. Patol. — 2001. — V.158. — P.1271-1278.
Crossref

6.Steinberg R. H. Research update: Report from a Workshop on Cell Biology of Retinal Detachment // Exp. EyeRes. — 1986. — Vol. 43, № 5. — P. 695-706.
Crossref

7.Steinberg P. J. Machemer R. Subretinal proliferation // Ophthalmol. — 1984. — Vol. 98, № 4. — P.456-462.
Crossref

8.Reichenbach A., Bringman A., Mueller cells in the Healthy and Diseased Retina // Springer, 2010. — p. 256.
Crossref

9.Linberg K. A., Sakai T., Levis G. P. et al. Experimental retinal detachment in the cone dominant ground squirrel: Morphology and basic immunocytochemistry // Vis. Neu-rosci. — 2002. — V.19. — P.603- 619.
Crossref

10.Wilson D. T., Green W. R. Histopathologic study of the effect of retinal detachment surgery on 49 eyes obtained post mortem // Am. J. Ophthalmol. - 1988. - Vol. 103, № 1. - P. 167- 179.
Crossref