Oftalmol Zh.2013;2:74-78
https://doi.org/10.31288/oftalmolzh201327478
Ultrastructural changes in the collagenic fibers of the corneal stroma, their organization and relation with the intercellular substance in different methods of treatment for obtaining the acellular corneal stroma
N. V. Pasechnikova, I. O. Nasinnik, N. F. Leus, N. E. Dumbrova, N. I. Molchanyuk
SI «Filatov Institute of Eye Diseases and Tissue Therapy of National Academy of Medical Sciences of Ukraine»
Key words: cornea, transplantation, experimental study
The conducted investigations showed that the developed method of treating the corneas obtained from the enucleated eyes including the two-moment influence of Triton X-100 and ammonium hydroxide before and after ultrasonic treatment allowed to obtain the acellular module of the corneal stroma with the smallest damage of the ultrastructure of the collagenic fibers.
References
1.Amano S. Decellularizing corneal stroma using N2 gas / S. Amano, N. Shimomura, S. Yokoo [et al.] // Mol. Vis. — 2008. — Vol. 14. — P. 878- 882.
2.Bader A. Tissue engineering of heart valves — human endothelial cell seeding of detergent acellularized porcine valves / A. Bader, T. Schilling, O. E. Teebken [et al.] // Eur. J. Cardiothorac. Surg. — 1998. — Vol. 14. — P. 279- 284.
Crossref
3.Grauss R. W. Decellularization of rat aortic valve allografts reduces leaflet destruction and extracellular matrix remodeling / R. W. Grauss, M. G. Hazekamp, S. van Vliet [et al.] // J. Thorac. Cardiovasc. Surg. — 2003. — Vol. 126. — P. 2003- 2010.
Crossref
4.He H. Use of high-pressure processing for oyster shucking and shelf-life extension / H. He, R. M. Adams, D. F Farkas [et al.] // J. Food. Sci. — 2002. — Vol. 67. — P. 640-645.
Crossref
5.Imanishi J. Growth factors: Importance in wound healing and maintenance of transparency of the cornea / J. Imanishi, K. Kamiyama, I. Iguchi [et al.] // Progr. Retin Eye Res. — 2000. — Vol. 19. — P. 113-129.
Crossref
6.Korossis S. A. In-vitro assessment of the functional per-formance of the decellularized intact porcine aortic root / S. A. Korossis, H. E. Wilcox, K. G. Watterson [et al.] // J. Heart Valve Dis. — 2005. — Vol. 14. — P. 408- 422.
7.Marquez S. P. Decellularization ofbovine cornea for tissue engineering applications / S. P. Marquez, V. S. Martirnez, W. M. Ambrose [et al.] // Acta Biomaterialia. — 2009. — Vol. 5. — P. 1839- 1847.
Crossref
8.Mertsching H. Engineering of a vascularized scaffold for artificial tissue and organ generation / H. Mertsching, T. Walles, M. Hofmann [et al.] // Biomaterials. — 2005. — Vol. 26. — P. 6610-6617.
Crossref
9.Meyer S. R. Comparison of aortic valve allograft decellularization techniques in the rat / S. R. Meyer, B. Chiu, T. A. Churchill [et al.] // J. Biomed. Mater. Res. A. — 2006. — Vol. 79. — P. 254-262.
Crossref
10.Oh J. Y. Processing porcine cornea for biomedical applications / J. Y Oh, M. K. Kim, H. J. Lee [et al.] // Tissue Eng. Part С Methods. - 2009. - Vol. 15, № 4. - P. 635¬645.
Crossref
11.Ohji M. Basement membrane synthesis by human corneal epithelial cells in vitro / M. Ohji, N. Sundar Raj, J. R. Hassell [et al.] // Invest Ophthalmol Vis Sci. - 1994. Vol. 35. - P. 479- 485.
12.Ota T. Novel method of decellularization of porcine valves using polyethylene glycol and gamma irradiation / T. Ota, S. Taketani, S. Iwai [et al.] // Ann Thorac. Surg. - 2007. - Vol. 83. - P. 1501-1507.
Crossref
13.Ponce M6rquez S. Decellularization of bovine corneas for tissue engineering applications / S. Ponce M6rquez, V. S. MartHnez, W. Mc Intosh Ambrose [et al.] // Acta Biomater. - 2009. - Vol. 5. - P. 1839-1847.
Crossref
14.Sasaki S. In vivo evaluation of a novel scaffold for artificial corneas prepared by using ultrahigh hydrostatic pressure to decellularize porcine corneas / S. Sasaki, S. Funamoto, Y Hashimoto [et al.] // Mol. Vis. - 2009. - Vol. 15. - P. 2022-2028.
15.Wu Z. The use of phospholipase A2 to prepare acellular porcine corneal stroma as a tissue engineering scaffold / Z. Wu, Y Zhou, N. Li [et al.] // Biomaterials. - 2009. - Vol. 30. - P.3513-3522.
Crossref
16.Xiao J. Construction of the recellularized corneal stroma using porous acellular corneal scaffold / J. Xiao, H. Duan, Z. Liu [et al.] // Biomaterials. - 2011. - Vol. 32. - P. 6962-6971.
Crossref
17.Zhang C. A comparative study on biocompatibility of acel-lular corneal stroma materials prepared by serial digestion methods. / С. Zhang, Y. Jin, Hu D. [et al.] // Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. - 2006. - Vol. 20. - P. 185-188.
18.Zhang C. Survival and integration of tissue-engineered corneal stroma in a model of corneal ulcer / C. Zhang, X. X. Nie, D. Hu [et al.] // Cell Tissue Res. - 2007. - Vol. 29. - P. 249-257.
Crossref