https://doi.org/10.31288/oftalmolzh201347280

Biochemical mechanisms of anticataractogenic action of preparations with antioxidant properties

N. F. Leus, S. G. Kolomiichuk, Nizar Boudaya Nizar, A. V. Girzheva, Yu. A. Zhuravok

SI «The Filatov Institute of Eye Diseases and Tissue Therapy NAMS of Ukraine»

Key words: cataract, antioxidants, glutathione, thiol groups, nicotinamide coenzymes.

In experiments on rabbits studied the effect of carotenoids and flavonoids on thiol system, of vitamin E and analog 2—4-methyl-3-pentenyl-6-acetoxy-2,5,7,8-tet-ramethylchroman on the content nicotinamide coenzymes of lens while modeling age-related cataracts.

It has been found that quercetin and lutein with zeaxanthin have a protective effect on the thiol system lens. Herbs mentioned above have had an stabilizing effect on the state of the thiol groups of proteins lens and level of reduced glutathione with experimental cataract. Application on a background light influence of preparations of vitamin E and analog instrumental in the substantial increase of level of the reduced forms of nicotinamide coenzymes in the lens of the eye, especially ratio of NADFH/NADF in the case of analogue of vitamin E.

References

1.Venger GYe, Ulyanova NA. Results of complex conservative treatment of patients with age-related cataract. Odesskii med. Zhurnal. 2005; 1: 52-4. Russian.

2.Veselovskaya ZF, Bobrova NF, Vit VV. Cataract. K.: Kniga plus; 2002. 208 p.

3.Kolomiichuk SG, Leus MF. Study of the mechanism of regulatory impact of vitamins on redox status of nicotinamide coenzymes and condition of the lens with the cataract factors. Visnyk morskoi meditsiny. 2012; 1: 49-51. Ukrainian.

4.Leus NF, Metelitsina IP, Oleinik TV et al. Role of vitamins and co-enzymes in degenerative diseases of the eye. Zhurnal AMN Ukrainy. 2005; 11(4): 737- 52. Russian.

5.Leus NF, Girzheva AV, Zhuravok YuA. Influence of biofla-vonoid (quercetin and routine) on development of patho-logical changes in the lens in modeling of age-related cataract. Oftalmol Zh. 2010; 6: 60-5. Russian.
Crossref

6.Leus NF, Budaya Nizar, Parkhomenko TV. Influence of ca-rotenoids on the stability of antioxidant enzymes and bio-physical properties of lens components while light energy exposing. Oftalmol Zh. 2012; 2: 54-7. Russian.
Crossref

7.Leus NF, Budaya Nizar. Efficacy of anticataract action of carotenoids (lutein and zeaxanthin) in the development of experimental cataract. Oftalmol Zh. 2012; 3: 64-7. Russian.

8.Leus MF, Metelitsyna IP, Drozhzhina GI, et al. Method of modeling of radiation cataract: Patent. 20178 Ukraine, PMK G 09B 23/28, № 4712831/SU; Appl. 13.07.89; Publ. 25.12.97; Bul., Prom. Vas.» № 6. Part. 2: 576.

9.Leus NF. Study of the biochemical mechanisms of cataract genesis. Glutathione levels in the development of experimental cataract. Oftalmol Zh. 1980; 7: 423- 6. Russian.

10.Metelitsyna IP, Kolomiichuk SG, Kuzmenko IV, Maltsev EV, Leus NF. On the possibility of slowing down the processes of lens accelerated aging with vitamin E derivative. Tsitologiia. 1997; 39(6): 490- 1. Russian.

11.Nasledov A. SPSS computer analysis of data in psychology and social sciences. StP.: Piter; 2005. 416 p.

12.New methods of biochemical analysis. Izd. Leningradsk-ogo univer; 1991. 395 p.

13.Pavlyuchenko KP, Mohhamed Zuheir Mahfuz Ibragim. Ef-fect of the thiol compounds level in the body on the lens stability to light exposure. Oftalmol Zh. 2004; 5: 57-61. Russian.

14.Rebrova OYu. Statistic analysis of medical data. Application of software package STATISTICA. Moscow: Media Sfera; 2003. 312 p.

15.Ulyanova NA. Thiol-disulfide status of the lens, chamber humidity and blood serum in cataract genesis (clinic ex-perimental study): author's thesis: 14.01.18. Ministry of Health of Ukraine, National Medical Academy of Post Graduate Study n/a P. L. Shupik. Kyiv; 2006. 20 p.

16.Shalk V. Lutein and zeaxanthin are two main components for eye health. Oftalmol Zh. 2010: 1: 108- 10. Russian.

17.Brigelius-Flohe R, Traber M VitaminE: function and me-tabolism. FASEB J. 1999; 13: 1145-55.
Crossref

18.Cao X-G, Li X-X, Bao Y-Z. Responses of human lens epi-thelial cells to quercetin and DMSO. Invest. Ophthalmol. Vis. Sci. 2007; 48: 3714-8.
Crossref

19.Cornish KM, Williamson G, Sanderson J. Quercetin me-tabolism in the lens: role in inhibition of hydrogen peroxide induced cataract. Free Rad. Biol. Med. 2002; 33(1): 63-70.
Crossref

20.Dorey CK, Granata L, Nichols CR. Dietary modulation of lens zeaxanthin in quail. Exp. Eye Res. 2005; 81: 464- 77.
Crossref

21.Fernandez MM, Afshari NA. Nutrition and the prevention of cataracts. Curr. Opin. Ophthalmol. 2008; 19(1): 66-70.
Crossref

22.Gao S, Qin T, Liu Z. Lutein and zeaxanthin supplementation reduces H2O2-induced oxidative damage in human lens epithelial cells. Mol. Vis. 2011; 17: 3180-90.

23.Giblin F, Reddy V. Pyridine Nucleotide in Ocular Tissues as Determined by the Cycling Assay. Exp. Eye Res. 1980; 31: 601-9.
Crossref

24.Giblin FJ. Glutathione: a vital lens antioxidant. J. Ocul. Pharmacol. Ther. 2000; 16: 121-35.
Crossref

25.Gupta SK, Trivedi D, Srivastava S. Lycopene attenuates oxidative stress induced experimental cataract development: an in vitro and in vivo study. Nutrition. 2003; 19: 794-9.
Crossref

26.Javadzadeh A, Ghorbanihaghjo A. Bonyadi S. Preventive effect of anion juice on selenite-induced experimental cataract. Indian J. Ophthalmol. 2009; 57: 185- 9.
Crossref

27.Jiang Q, Cao C, Zhou C. Quercetin attenuates UV-and H2O2-induced decrease of collagen type I in cultured human lens epithelial cells. J. Ocul. Phar. Ther. 2008; 24(2): 164- 70.
Crossref

28.Kay CD. The future of flavonoid research. Brit. J. Nutr. 2010; 104: S91-S95.
Crossref

29.Krinsky NI, Landrum JT, Bone RA. Biologic mechanisms of the protective role of lutein and zeaxanthin in the eye. Ann. Rev. Nutr. 2003; 23: 171- 201.
Crossref

30.Li L, Duker JS, Yoshida Y. Oxidative stress and antioxidant status in older adults with early cataract. Eye. 2009; 23: 1464-8.
Crossref

31.Majumdar S, Srirangam R. Potential of the bioflavonoids in the prevention/treatment of ocular disorders. J. Pharm. Pharmacol. 2010; 62(8): 951-65.
Crossref

32.Moeller SM, Voland R, Tinker L. Associations between age-related nuclear cataract and lutein and zeaxanthin in the diet and serum in the carothenoids in age-related eye disease study, an ancillary study of the women's health initiative. Arch. Ophthalmol. 2008; 126(3): 354- 64.
Crossref

33.Myhrstad MCW, Carlsen H, Nordstrom O. Flavonoids in-crease the intracellular glutathione level by transactivation of the y-glutamylcysteine synthetase catalytical subunit promoter. Free Rad. Biol. Med. 2002; 32: 386- 93.
Crossref

34.Sanderson J, McLauchlan WR, Williamson G. Quercetin inhibits hydrogen peroxide-induced oxidation of the rat lens. Free Rad. Biol. Med. 1999; 26(5/6): 639- 45.
Crossref

35.Vu HTV, Robman L, Hodge A. Lutein and zeaxanthin and the risk of cataract: the melbourne visual impairment project. Invest. Ophthalmol. Vis. Sci. 2006; 47: 3783- 6.
Crossref