https://doi.org/10.31288/oftalmolzh201343034

Prediction of invasion sclera uveal melanoma using molecular markers lymphocyte activation

L. Velichko, V. V. Vit, A. P. Malecki, E. Dragomiretskaya

 SI «The Filatov Institute of Eye Diseases and Tissue Therapy NAMS of Ukraine»

Key words: uveal melanoma scleral invasion, molecular markers of lymphocyte activation, ROC analysis, logistic regression.

The purpose. To study the predictability of uveal melanoma scleral invasion with molecular markers of lymphocyte activation.

Material and methods. A comparative analysis of the expression of lymphocyte activation molecular markers was made in patients with uveal melanoma with tumor invasion into the sclera (84patients) and without scleral invasion (173 patients). The level of expression of molecular markers of lymphocyte activation was deter-mined using monoclonal antibodies by histoimmunochemical method. 

Results. A study of the expression level of lymphocyte activation markers was made in patients with uveal melanoma with and without scleral invasion. Uveal melanoma patients with scleral invasion had a violation of hematoophthalmic barrier, which led to the activation of immune cells and significant increase in the expression of molecular markers of activated lymphocytes SD7 + SD25 + SD38 + SD45 + SD54 + SD95 + SD150 + in comparison to patients without invasion. The study evaluated predictability of lymphocyte activation molecular markers for predicting uveal melanoma scleral invasion. ROC analysis showed that the most informative markers were CD95+ and CD54+. Thresholds expression level was defined on peripheral blood lymphocytes SD95 + > 325 cells / uL and SD54 + > 19% enabling to predict (80%) germination of uveal melanoma in the sclera.

References

1.Vit VV. Tumor pathology of the vision. Odessa: Astroprint; 2009. 610 p.

2.Vit VV. Pathological anatomy and medical pathomorpho-sis of pigmented neoplasms of h uman eye uveal tract: author's abstract. Doctor of Med. Sc. Pathologic Anatomy. Odessa; 1987. 30 p.

3.Male D, Brostoff J, Dig DB. Immunology. M.: Logosfera; 2007. 555 p.

4.Engelhardt B, Ransohoff RM. The ins and outs of T-lymphocyte trafficking to the CNS: anatomical sites  and molecular mechanisms. Trends Immunol. 2005; 26: 485-95.
Crossref

5.Greenwood J, Begley DJ. New Concepts of a blood-brain. New York, London: Plenum Press; 1995. 331 p.
Crossref

6.Zhang X, Brunner T, Carter L et al. Unequal death in T-helper cell (Th)1 and Th2 effectors: Th1, but not Th2, effectors undergo rapid Fas/FasL-mediated apoptosis. J. Exp. Med. 1997; 185(10): 1837-49.
Crossref