https://doi.org/10.31288/oftalmolzh201427177

Experimental research of the influence of seven-day tamponade with perfluorine-organic compounds on the ultrastructure of the rabbit eye

D. V. Zhmurik, M. V. Milienko

Kyev City Clinical Eye Hospital «Center Introduction of Eye Microsurgery, Kyev (Ukraine)

Perfluorine-organic compounds (PFOC) possess valuable qualities for  vitreoretinal surgeries, and their use for short-term tamponade could expand indications to operative treatment and improve results. However, there is no unequivocal opinion concerning mechanical action of PFOC on the ultrastructure of the retina.

Purpose. To study the influence of 7-day tamponade with PFOC on the ultra-structure of the eye retina of the rabbit in experiment; comparison of the effect of PFOC, a physiological solution and « light» silicone oil with viscosity of5700 cCm in dynamics by carrying out electron-microscopic study (EMS) on different terms after end tamponade

Material and methods. The experimental research has been carried out on 12 rabbits (24 eyes). All animals were performed posterior closed subtotal vitrectomy with the subsequent 7-day tamponade with PFOC (the right eye), «light» silicone and physiological solution (the left eye). EMS was made after the end of tamponade in 7, 14 and 30 days.

Results. Ultrastructural changes caused in the retinal elements revealed both under the influence of PFOC and silicone, are similar to each other. In reply to seven-day tamponade of the vitreal cavities with the applied substances, the structure of the retina react the same, mainly by hydroptic changes of elements of the smooth endoplasmatic network and mitochondria.

Conclusion. After seven-day tamponade with the applied substances, structures of  the retina react with the same changes. However, these changes are related to the category of reactive but not damaging and have reversible character. PFOC can be considered as candidates for carrying out short-term tamponade.

Key words: retina, ultrastructure, perfluorine-organic Compounds (perfluorocarbon liquid), light silicone.

References

1.Shkvorchenko DO. Complex surgical treatment of retinal detachment complicated by giant tears and detachments from the dentate line with liquid perfluoroorganic compounds: author's thesis for Candidate of Med. Science: 14.00.08. M.; 1995. 132 p.

2.Shkvorchenko DO, Kashtan OV, Makarov KN, Ronkina TI. IIlKBopqeHKO ?. O. Experimental — clinical rationale for the use of short-term postoperative tamponing in vitreoret-inal surgery. Perfluoroorganic compounds in biology and medicine: collection of papers. Pushchino; 1999. 186- 92.

3.Shkvorchenko DO, Levina LV. On tactic for surgical treat-ment of proliferative diabetic retinopathy complicated by anterior proliferative vitreoretinopathy. Oftalmokhirurgiia. 2006;1:29- 32. Russian.

4.Chang S, Sparrow JR, Iwamoto T, Gershbein A, Ross R, Ortiz R. Experimental studies of tolerance to intravitreal perfluoro-n-octane liquid / // Retina. 1991;11:367- 74. 
Crossref

5.Clark LC Jr, Gollan F. Survival of mammals breathing organic liquids equilibrated with oxygen at atmospheric pressure. Science. 1966;152:1755- 6.
Crossref

6.Devin F, Jourdan T, Saracco JB, Lucciani A. Experimental tolerability of perfluorodecalin in prolonged intraocular tamponade. J Fr Ophtalmol. 1995;18:268- 74.

7.Drury B, Bourke RD. Short-term intraocular tamponade with perfluorocarbon heavy liquid. BrJOphthalmol. 2010:694- 8.
Crossref

8.Flores-Aguilar M, Munguia D, Loeb E, Crapotta JA, Vuong C, Shakiba S, Bergeron-Lynn G, Wiley CA, Weers J, Freeman WR. Intraocular tolerance of perfluorooctylbro-mide (perflubron). Retina. 1995; 15: 3-13.
Crossref

9.Haidt SJ, Clark LC, Ginsberg J. Liquid perfluorocar¬bon replacement of the eye. Invest. Ophthalmol. Vis. Sci. 1982;22:233-8.

10.Norman HJ. Requirements of bioethics of the Helsinki declaration about ethical regulation of medical researches. Chronicles of WHO. 1985;39(3):3-9.

11.Orzalesi N, Migliavacca L, Bottoni F, Miglior S. Experimental short-term tolerance to perfluorodecalin in the rabbit eye: a histopathological study. Curr Eye Res. 1998;17:828- 35.
Crossref

12.Reynoldes ES. The use of lead citrate at higt pH an electronopaque stain in electron microscopy. J. Cell Biol;17:208- 12.
Crossref

13.Sirimaharaj M, Balachandran C, Chan WC, Hunyor AP, Chang AA, Gregory-Roberts J, Hunyor AB, Playfair TJ. Vitrectomy wih short term postoperative tamponade using perfluorocarbon liquid for giant retinal tears. Br J Ophthalmol. 2005;89;1176-9.
Crossref

14.Terauchi H, Okinami S, Kozaki Z, Tanihara H, Nagata M, Segawa Y. Experimentalstudy on the effects of a replacement of the vitreous body with perfluorotributylamine on the rabbiteye. Nihon Ganka Gakkai Zasshi. 1989;93:294- 301.