https://doi.org/10.31288/oftalmolzh201429296

Some peculiarities of carbohydrate metabolism in the cornea and metabolic correction of its disturbances in modeled alkaline burn of the cornea

R. I. Chalanova, T. V. Parkhomenko, M. Mbarki

State Institution The Filatov Institute of Eye Diseases and Tissue Therapy of the NAMS of Ukraine, Odessa, (Ukraine)

Key words: eye burns, lactate, Pyruvate, cytoflavin

Introduction. On the basis of the content of the substrates of glycolysis of pyruvate and lactate in the burnt cornea the conclusion may be made about the way of realization of the tissue respiration in the eye burn and efficacy of using cytoflavin with the antihypoxic aim.

Purpose of the study. To reveal disorders of the anaerobic-aerobic phases of the carbohydrate oxidation in the burn trauma of the cornea (by the level and ratio of lactate and pyruvate) and possibility of the correcting influence of cytoflavin on the pathochemical processes.

Materials and methods. The study was made in both eyes of 20 rabbits with modeled severe alkaline burn. The controls were intravenously introduced 2.5 ml of the physiological solution from 10 to 14 days; the main group was introduced 0.5 ml of cytoflavin and 2.0 ml of the physiological solution. The cornea was taken for the biochemical investigation on the 3rd, 7th, 21st, 30th and 45th day.

Results. The data obtained are evidence of disorder of the aerobic type of the tissue respiration. The application of cytoflavin contributes to improvement of the course of the pathochemical processes in the burnt cornea. The difference in the pyruvate and lactate content on the 45th day of the burn process was 1.5 % in the main group of animals and 10 % — in the controls.

Conclusion. There was established suppression of the aerobic phase of the carbohydrate metabolism and improvement of its indices after the application of intravenous injections of cytoflavin with the antihypoxic aim.

References

1.Birich TV. Eye burns. Minsk:Belarus; 1979. 173 p.

2.Volkov VV. On elements of eye burn disease pathogenesis. Simposium in treatment of eye burns. Theses. M.; 1989. 3-4.

3.Lukyanova LD. The role of bioenergetic disorders in hypoxia pathogenesis. Patologicheskaia fisiologiia I eksperimentalnaia terapiia. 2004;2:2-11. Russian.

4.New methods of biochemical analyses. Izd. Leningradskogo univer. 1991. 395 p.

5.Puchkovskaia NA, Yakimenko SA, Nepomyashchaia VM. Eye Burns. M.: Meditsina; 2001. 256 p.

6.Rebrova OYu. Statistic analysis of medical data. Applying software package STATISTICA. M.:Media Sfera; 2002. 312 p.

7.Chalanova RI, Kolomiichuk SG, Mbaraki M. Investigation of the activity of redox enzymes (lactate dehydrogenase and glucose-6-phosphate dehydrogenase) in chamber liq¬uid in treatment of experimental corneal burn with intravenous cytoflavin. Collection of scient. Papers. Problemy voiskovoi okhorony zdorovya. Kyiv. 2013;1(38):404- 16.

8.Chalanova RI, Kolomiichuk SG, Mbaraki M. Lactate de-hydrogenase and glucose-6-phosphate in the blood in experimental eye burns in the application of intravenous cytoflavin. Oftalmol Zh. 2013;4:81-6. Ukrainian.
Crossref

9.Chalanova RI, Kolomiichuk SG, Baidan EI, Mbaraki M. ATP content, and glucose-6-phosphate in simulated cornea alkali burn, and the possibility of its correcting by cytoflavin. Oftalmol Zh. 2013;5:78- 83. Russian.

10.Bergmeyer HU. Methoden der enzymatischen Analyse. Berlin. 1986. S.2198- 2203.

11.Brodovsky SC, NcCarty CA. Management of alkali burns. An 11 year retrospective review. Ophthalmol. 2000;107:1829-35.
Crossref   Pubmed

12.Kuckelkorn R, Schrage N, Keller G. Emergency treatment of chemical and thermal eye burns. Acta Ophthalmol. Scand. 2002;80:4-10.
Crossref   Pubmed