https://doi.org/10.31288/oftalmolzh201543843

Effect of ocular hypertension on the levels oflipid peroxidation products in anterior eye tissues in experimental diabetes

V.R. Yurevych, Cand. Sc. (Med), Ass. Prof.

Danylo Halytsky Lviv National Medical University

E-mail: yurevych@yahoo.com

 

Background: Even though there has been significant advance in the care of ocular complications of diabetes mellitus, the issue related to the prevention of and therapy for this pathology is still an important area that needs to be addressed.

Purpose: To investigate the effect of ocular hypertension on the levels oflipid peroxidation products in anterior eye tissues in experimental diabetes.

Materials and Methods: Thirty-two rabbits were divided into four groups: group 1 (controls; 10 rabbits), group 2 (diabetes and ocular hypertension (D+OH) group; 8 rabbits), group 3 (diabetes-only group; 7 rabbits) and group 4 (ocular hypertension-only group; 7 rabbits). In these animals, the levels of malondialdehyde and diene conjugate in the anterior chamber angle tissue and aqueous humor were determined.

Results: The activation of lipid peroxidation was seen as increased diene conjugate andmalondialdehyde levels in the anterior chamber angle tissue and aqueous humor in animals with diabetes. In D+OH group, the levels of malondialdehyde and diene conjugates in the anterior chamber angle tissue, and the levels of malondialdehyde and diene conjugates in the aqueous humor were 23.8%, 21.1%, 31.9% and 28.1%, respectively, higher than those in diabetic animals without ocular hypertension.

Conclusion: Increased lipid peroxidation imbalance found in anterior eye tissues and aqueous humor in animals with diabetes mellitus and experimental ocular hypertension is a key element of the mechanism of the accelerated destruction in the aqueous humor outflow pathways.

Keywords: ocular hypertension, experimental diabetes, anterior eye, lipid peroxidation.

References

1. Gatsu MV, Somov EE.  [Glaucoma in patients with diabetes mellitus]. In: Balashevich LI, Izmailov AS, editors. [Diabetic ophthalmopathy]. St. Petersburg: Chelovek; 2012. Chapter 12.  Russian.

2. Lipatov DV, Chistyakov TA, Kuzmin AG. [Diabetic glaucoma: clinical and treatment features]. Endokr Khir. 2011;1:21-6. Russian.
Crossref

3. Kramorenko IuS. [Clinical and biochemical aspects of the pathogenesis of primary glaucoma]. [Dr. Sc. (Med) Thesis Abstract]. Moscow; 1992. 38 p. Russian.

4. Sommer A. Intraocular pressure and glaucoma. Am J Ophthalmol. 1989 Feb 15;107(2):186-8. 
Crossref   Pubmed

5.Bunin AIa, Babizhaev MA, Suprun AV.[Participation of the lipid peroxidation process in the destruction of the drainage system of the eye in open-angle glaucoma].Vestn Oftalmol. 1985 Mar-Apr;101(2):13-6. Russian.

6. Kravchuk EA. [Free-radical oxidation in the pathogenesis of eye diseases]. Vestn Oftalmol. 2004 Sep-Oct;120(5):48-51. Russian.

7. Ahmed FN, Naqvi FN, Shafiq F. Lipid peroxidation and serum antioxidant enzymes in patients with type 2 diabetes mellitus. Ann N Y Acad Sci. 2006 Nov;1084:481-9.
Crossref   Pubmed

8. Baynes JW. Role of oxidative stress in development of complications in diabetes.Diabetes. 1991 Apr;40(4):405-12.
Crossref   Pubmed

9. Kesavulu MM, Rao BK, Giri R et al.  Lipid peroxidation and antioxidant enzyme status in Type 2 diabetics with coronary heart disease. Diabetes Res Clin Pract. 2001 Jul;53(1):33-9.
Crossref   Pubmed

10. Navajas EV, Martins JR, Melo LA Jr et al. Concentration of hyaluronic acid in primary open-angle glaucoma aqueous humor. Exp Eye Res. 2005 Jun;80(6):853-7.

11. Sacc? SC, Pascotto A, Camicione P et al.  Oxidative DNA damage in the human trabecular meshwork: clinical correlation in patients with primary open-angle glaucoma. Arch Ophthalmol. 2005 Apr;123(4):458-63.

12. Birich TV, Birich TA, Marchenko LN, Remizonova DB, Fedulov AS. [Lipid peroxidation in the blood of primary glaucoma patients]. Vestn Oftalmol. 1986 Jan-Feb;102(1):13-5. Russian.

13. Burlakova EB, Khrapova NG. [Membrane lipid peroxidation and natural antioxidants]. Usp Khim. 1985;54(9):1540-58. Russian.

14. Serdyuk VN. [Investigation of the levels of lipid peroxidation products in the retina and optical nerve in experimental glaucoma]. Khark Surg School. 2011;6(51):80-3. Russian.

15. Dadali VA. [Peroxidation processes in the body and natural antioxidants]. In: Gichev IuP, Oganova E, editors. [Introduction in special micronutrientology]. Novosibirsk: Meditsina; 1999. p240-63. Russian.

16. Feillet-Coudray C, Rock E, Coudray C et al. Lipid peroxidation and antioxidant status in experimental diabetes. Clin Chim Acta. 1999 Jun 15;284(1):31-43.

17. Wang J, Wan R, Mo Y et al. Creating a long-term diabetic rabbit model. Exp Diabetes Res. 2010;2010:289614.

18. Zhu MD, Cai FY. Development of experimental chronic intraocular hypertension in the rabbit. Aust N Z J Ophthalmol. 1992 Aug;20(3):225-34.

19. Padminikedar, Chakrabarti CM. Effect of Jambolan seed treatment on blood sugar, lipids and urea in streptozotocin induced diabetes in rabbits. Indian J Physiol Pharmacol. 1983 Apr-Jun;27(2):135-40.

20. New methods of biochemical analysis. Leningrad: Leningrad University Publishing House; 1991. 395 p. Russian.

21. Nasledov  AD. [SPSS. Computer data analysis in psychology and social science]. St. Petersburg: Piter; 2005. 416 p.  Russian.

22. Mikheytseva IN, Kashintseva LT, Lipovetskaya EM, Kopp OP. [Lipid peroxidation: initiation with adrenalin and blocking with verapamil in adrenalin-induced glaucoma model]. In: [Proceedings of the 1st Russian Pathophysiology Congress]; 1996 Oct 19-19; Moscow. p.196. Russian.