https://doi.org/10.31288/oftalmolzh201553034

The level of interferons ? and ? in the blood of patients with uveitis of different clinical characteristic and course of the disease

N.V. Konovalova

Chaibi Abderrahim 

Filatov Institute of Eye Diseases and Tissue Therapy,

Odessa, (Ukraine)

Introduction. Social importance of uveitis is determined by high frequency of occurrence, affection of persons mainly of work ability age, high frequency of disability due to vision.

Objective. To determine the peculiarity of interferons ? and ? expressions in the blood of patients with uveitis of different clinical characteristic and course of the pathological process.

Materials and methods. There were examined 61 patients with uveitis of different localization (anterior and posterior), clinical course (chronic and acute), degree of severity (severe and moderate) and differential diagnosis (iridocyclitis, focal and disseminated chorioretinitis, neuritis, panuveitis) who were examined ophthalmoscopically (ophthalmoscopy, tonometry, visometry). 59 people were determined the level of interferons ? and ? in the blood plasma by the method of the immune enzyme analysis. 

Results. There was determined peculiarity of interferons ? and ? expressions in the blood of patients with uveitis of different clinical characteristic and course peculiarities of the pathological process. It is shown that the content of these cytokines are significantly lower in posterior uveitis as well as in the chronic course with high degree of reliability. There was found a reliable correlation between the level of interferons under the study and above mentioned characteristics. 

The degree of uveitis severity does not correlate with the level of interferons in the patients’ blood as well as there was no revealed association between the average values of concentration of interferons ? and ? in the blood of the patients with uveitis taking into account the differential diagnosis.

Conclusions. The data obtained prove the role of interferons ? and ? in development of uveitis and may be of interest for determining the character of the course of the pathological process and treatment efficacy.

Key words: uveitis, clinical characteristic, course, interferons ? and ?, blood plasma

Literature

1.Vorobieva AA. [Medical microbiology, virology and Immunology atlas]. M.: MIA. 2003; 195. Russian.

2.Bakirov AB, Shagalina AU, Fukalova LA. In: Bakirov AB., Shagalina AU., Fukalova LA. Tsitoksiny i vospalenie. 2002; 2: 92. Russian.

3.Vasilyeva IA, Jchahov AV, Trofimov AV. In: Vasilieva IA, Jchahov AV, Trofimov AV. Tsitoksiny i vospalenie. 2003; 2: 51-57. Russian.

4.Glanz S. [Biomedical statistics]: translation from English. M.: Practice; 1998. 459. Russian.

5.Ershov FI. [Interferon system in normal and pathological condition]. M.: Meditsina; 1996. 72. Russian.

6.Zaitseva GA, Vershynina OA, Matrohina OI, Senkina EA, Karpova MV. [Cytokine status of blood donors and its components]. Fundamentalnye issledovaniya. 2011; 3: 61-65. Russian.

7.Zmyzgova AV. [Interferon therapy of viral hepatitis]. M: 1999; 234. Russian.

8.Instruction for using the kit of reagents for quantitative determination of human alpha interferon in biological fluids and human culture mediums. ZAO "Vector-Best'': Novosibirsk; 30.05.08. 22. Russian.

9.Instruction for using the kit of reagents for quantitative determination of human gamma interferon in biological fluids and human culture mediums. ZAO "Vector-Best'': Novosibirsk; 30.05.08. 22. Russian.

10.Katargina LA, Hvatova AV. [Endogenous uveitis in children and adolescents]. M.: Meditsina; 2000. 320.

11.Katsnelson LA., Tankovskyy VE. [Uveitis]. M.: 4 filial Voenizdata; 1998. 208. Russian.

12.Konovalova NV, Abderrahim Shaibi. [Cellular and humoral immunity in patients with uveitis]. Oftalmol. Zh. 2015; 2: 34-36. Russian.

13.Kuznetsov SI, Latash VG. Meditsinskaya immunologiya. 2002; 2: 364-365. Russian.

14.Makarov OV, Bachareva IV. Fundamentalnye issledovaniya.  М.: Akademiya Yestestvoznania. 2004; 1: 67.

15.Mamaev SN. Klinicheskaya i laboratornaya diagnostika. 2001; 8: 45-47. Russian.

16.Neverova EA. Pathogenetic substantiation of ozone use in endogenous uveitis. In: Dissertation. of Candidate of Medical Sciences 14.00.16 Patologicheskaya fiziologiya. 2007; Saransk: 116. Russian.

17.Riabicheva TG, Varaskin NA, Timofeeva NV. Rukavishnikov MJ. Determination of cytokines with the use of enzyme immunoassay method ZAO Vector-Best. Inform. bulleten Novosti "Vector-Best”. 4 (34). December 2004 Available at: http://www.vector-best.ru/nvb/n34/st34_4.htm. Russian.

18.Tsaregorodtseva TM, Serova TI. Cytokines in Gastroenterology. M.: Anakharsis; 2003. 50. Russian.

19.Razavi NL. Proinflammatory cytokines in response to insulin-induced 

20.hypoglycemic stress in healthy subjects. Metabolism. 2009; 58 (4): 443-448. 
Crossref   Pubmed

21.Fields Virology / ed. David M. Knipe. Philadelphia: «Lippincott Williams & Wilkins». 2001; 1: 321–353.

22.Grau G, Roux-Lombard P, Gyster C. Immunol. 1989; 68: 196–198.

 

23.The Cytokine Handbook. Ed. Thomson AW and Lotze MT. London, San Diego: Academic Press. 2003.