J.ophthalmol.(Ukraine).2018;4:49-55.
https://doi.org/10.31288/oftalmolzh201844955
Received: 18 May 2018; Published on-line: 31 August 2018
Structure of the chorioretinal complex in the rabbit eye after vitrectomy. Report 2. Vitreous cavity irrigation with different temperature solutions for 60 minutes
O.S. Zadorozhnyy1, Cand Sc (Med), R.E. Nazaretian1, V.V. Myrnenko1, V.A. Naumenko1, Dr Sc (Med), Prof., E.V. Maltsev1, Dr Sc (Med), Prof., N.V. Pasyechnikova1, Dr Sc (Med), Prof., V.V. Shafranskii2, Dr Sc (Med), Ass. Prof.
1 Filatov Institute of Eye Diseases and Tissue Therapy, NAMS of Ukraine; Odessa (Ukraine)
2 Bohomolets National Medical University; Kiev (Ukraine)
E-mail: laserfilatova@gmail.com
TO CITE THIS ARTICLE: Zadorozhnyy OS, Nazaretian RE, Myrnenko VV, Naumenko VA, Maltsev EV, Pasyechnikova NV, Shafranskii VV. Structure of the chorioretinal complex in the rabbit eye after vitrectomy. Report 2. Vitreous cavity irrigation with different temperature solutions for 60 minutes. J.ophthalmol.(Ukraine).2018;4:49-55. https://doi.org/10.31288/oftalmolzh201844955
Background: It remains poorly understood what should be the temperature of the irrigation solution for intraocular surgery and how long it is reasonable to use irrigation solutions during vitrectomy.
Purpose: To investigate the structure of rabbit chorioretinal complex after vitrectomy with the use of irrigation solutions having different temperatures for 60 minutes.
Materials and Methods: Twelve Chinchilla rabbits (24 eyes) were divided into two experimental groups and one control group. The two experimental groups, each of 5 rabbits (10 eyes), underwent a three-port pars plana vitrectomy with either 22°C or 5°C irrigation solution. Two intact rabbits (4 eyes) were used as controls for comparison. The duration of irrigation/aspiration was 60 minutes. Specimens for histology were obtained at days 1 and 7 after surgery. The chorioretinal complex (i.e., the choroid and retina) was histologically examined.
Results: After vitrectomy with 60-minute continuous vitreous cavity cooling with either the 22°C or 5°C irrigation solution, we found retinal structural changes in the form of uneven edema, (a) predominantly in the inner retinal layers, at day 1, and (b) in both the inner and outer retinal layers, at day 7. There were destructive changes in the inner retinal layers on some slides from rabbits of both groups. In addition, the choroid appeared somewhat thickened.
Conclusion: In the rabbit eyes after vitrectomy with 60-minute continuous vitreous cavity cooling with either the 22°C or 5°C irrigation solution, we found the uneven retinal edema that is likely to be mediated by aseptic inflammatory response to cooling-induced and mechanical damage to the retina, and might be also associated with the composition of the irrigation fluid.
Keywords: vitrectomy, intraocular temperature, rabbit eye, chorioretinal complex
References
- Machemer R, Parel JM, Norton EW. Vitrectomy: a pars plana approach. Technical improvements and further results. Trans Am Acad Ophthalmol Otolaryngol. 1972 Mar-Apr;76(2):462-6
- Postel EA, Pulido JS, Byrnes GA, et al. Long-term follow-up of iatrogenic phototoxicity. Arch Ophthalmol. 1998 Jun;116(6):753-7
Crossref Pubmed - Farah M, Maia M, Rodrigues EB. Dyes in Ocular Surgery: Principles for Use in Chromovitrectomy. Am J Ophthalmol. 2009 Sep;148(3):332-40
Crossref Pubmed - AbdEl Dayem H, Hartzer M, Williams G, et al. The Effect of Vitrectomy Infusion Solutions on Postoperative Electroretinography and Retina Histology. BMJ Open Ophth. 2017; 1:e000004.doi:10.1136/bmjophth-2016-000004
Crossref Pubmed - Hasumura T, Yonemura N, Hirata A, et al. Retinal Damage by Air Infusion during Vitrectomy in Rabbit Eyes. Invest Ophthalmol Vis Sci. 2000;41:4300–04
- Rossi T, Querzoli G, Angelini G, et al. Ocular perfusion pressure during pars plana vitrectomy: a pilot study. Invest Ophthalmol Vis Sci. 2014 Dec 2;55(12):8497-505
Crossref Pubmed - Alzaga AG, Cerdan M, Varon J. Therapeutic hypothermia. Resuscitation. 2006 Sep;70(3):369-80
Crossref Pubmed - Polderman KH, Herold I. Therapeutic hypothermia and controlled normothermia in the ICU: Practical considerations, side effects, and cooling methods. Crit Care Med. 2009; 37: 1101–20
Crossref Pubmed - Hypothermia after Cardiac Arrest Study Group. Mild therapeutic hypothermia to improve the neurologic outcome after cardiac arrest. N Engl J Med. 2002;346:549–56
Crossref Pubmed - Yenari MA, Han HS. Neuroprotective mechanisms of hypothermia in brain ischaemia. Nat Rev Neurosci. 2012 Feb 22;13(4):267-78
Crossref Pubmed - Iguchi Y, Asami T, Ueno S, et al. Changes in vitreous temperature during intravitreal surgery. Invest Ophthalmol Vis Sci. 2014 Apr 11;55(4):2344-9
Crossref Pubmed - Zadorozhnyy OS, Nazaretian RE, Myrnenko VV, Naumenko VA, Pasyechnikova NV. [Experimental study of the epibulbar and intraocular temperature in the rabbit under hypothermic conditions]. Oftalmologiia. Vostochnaia Evropa. 2018;1:73-81. Russian
- Zilis JD, Chandler D, Machemer R. Clinical and Histologic Effects of Extreme Intraocular Hypothermia. Am J Ophthalmol. 1990 Apr 15;109(4):469-73
Crossref Pubmed - Anatychuk LI, Pasyechnikova NV, Zadorozhnyy OS, et al. [A thermoelectric device for measuring intraocular temperature]. Thermoelektrika. 2015;3:31-40. Ukrainian
- Anatychuk LI, Pasyechnikova NV, Zadorozhnyy OS, et al. [A thermoelectric device for measuring intraocular temperature]. Oftalmol Zh. 2015;6:50-3. Russian
Crossref Pubmed - Zhmurik DV, Vit VV, Dumbrova NE, et al. [Structural changes of the rabbit retina after 14-days tamponade of the vitreal cavity with perfluoro-organic compounds]. Oftalmol Zh. 2015;5:52-7. Russian
Crossref - Zadorozhnyy OS, Nazaretian RE, Myrnenko VV, Naumenko VA, Maltsev EV, Pasyechnikova NV. Structure of the chorioretinal complex in the rabbit eye after vitrectomy. Report 1. Vitreous cavity irrigation with different temperature solutions for 30 minutes. J. Ophthalmol.(Ukraine). 2018;3:73-84.
Crossref