http://doi.org/10.31288/oftalmolzh201864551

Received: 11 September 2018; Published on-line: 31 December 2018

Correlation between axial length and anterior chamber depth of the eye and retinal disorders in type 2 diabetic rabbits with myopia

Mohammad Abdulhadi, Cand. Med. Sc.; I.N. Mikheytseva, Dr. Biol. Sc.; A.A. Putienko, Dr. Med. Sc.;  A.G. Kovalchuk, Cand. Med. Sc.; S.G. Kolomiichuk, a research fellow; T.I. Siroshtanenko, a junior research fellow

Filatov Institute of Eye Diseases and Tissue Therapy, NAMS of Ukraine; 

Odessa (Ukraine) 

E-mail: filatovbiochem@ukr.net

TO CITE THIS ARTICLE: Mohammad Abdulhadi, Mikheytseva IN, Putienko AA, Kovalchuk AG, Kolomiichuk SG, Siroshtanenko TI. Correlation between axial length and anterior chamber depth of the eye and retinal disorders in type 2 diabetic rabbits with myopia. J.ophthalmol.(Ukraine).2018;6:44-51. http://doi.org/10.31288/oftalmolzh201864551  

 

Background. Recently, a protective effect of high myopia on the retina in diabetes has been reported; however, the mechanism of the interrelation between the elongation of axial length and the severity of diabetic complications in the retina is unclear.

Purpose. To compare retinal changes in experimental type 2 diabetes melitus (T2DM) in animals with and without myopia as well as to determine the correlation betweeen axial length and anterior chamber depth of the eye and the severity of retinal disorders in DM.

Material and Methods. Eyelids of two-week animals (30 rats) were sutured to induce axial myopia [12]. The animals were kept under poor light conditions for 14 days in order to achieve a higher rate of myopia [11, 13]. After a fortnight, the sutures were removed. After another fortnight, 15 rats with experimental myopia and 15 intact rats were modelled T2DM. T2DM was induced using 5 daily intraperitoneal injections of streptozotocin (15.0 mg per 1 kg). Control group comprised 10 intact rats which were kept under natural light condition for 14 days. All the animals were performed ultrasound scanning of the anterior segment to determine axial length (AL) and anterior chamber depth (ACL). A criterion of a diabetes onset was a blood glucose level of 4.5 mmol/l and above. The state of the retina was assessed ophthalmoscopically and expressed using a four-point scoring system. After two months, the animals were sacrificed under general anesthesia and the eyeballs were enucleated. Axial length was measured post mortem using a digital sliding caliper (Topex). Data obtained were processed using the non-parametric tests.

Results. In experimental axial myopia, axial length increased by 20.5% compared with control. Experimental type 2 diabetes mellitus did not influence significantly on the axial length of the animals. In the rats with type 2 diabetes and deprivation myopia, axial length was significantly increased by 21.9% and by 19.4% as compared with control and the diabetic-only rats, respectively. Anterior chamber depth in the rats with type 2 diabetes mellitus and deprivation myopia was also increased compared with control. In experimental streptozotocin-induced type 2 diabetes mellitus, the diabetic rats with deprivation myopia had more pronounced changes in the retinal vessels as compared with the non-diabetic rats with deprivation myopia. A negative correlation was revealed between axial length values and the state of the retina in the diabetic rats with deprivation myopia (RSpearman  = - 0.68, р<0.05); in a part of the animals, with an increase in axial length, the severity of the pathologic changes in the retina was less pronounced.    

Conclusion. In our experiment, the myopisation of the eyeball, including the elongation of axial length and the deepening of anterior chamber depth, is accompanied by a decrease of the signs of vascular disorders in the retina which are common for DM. This is also evidenced by the presence of a negative correlation between the myopisation of the eyeball and diabetes-associated disorders in the ocular fundus of the rats. 

Keywords: deprivation myopia, type 2 diabetes mellitus, retina, ultrasound testing, rats, experiment 

 

References

1.Balashevich LI, Izmailova AS. [Diabetic Ophthalmology]. S.-Petersburg SPB:Chelovek; 2012. 396p. Russian.

2.Tayyab H, Haider MA, Haider Bukhari Shaheed SA. Axial myopia and its influence on diabetic retinopathy. J. Coll. Physicians Surg. Pak. 2014 Oct;24(10):728-31. doi: 10.2014/JCPSP.728731.

3.Wang X, Tang L, Gao L, Yang Y., Cao D, Li Y. Myopia and diabetic retinopathy: A systematic review and meta-analysis. Diabetes Res Clin Pract. 2016 Jan;111:1-9. doi: 10.1016/j.diabres.2015.10.020. Epub 2015 Oct 23.
Crossref   Pubmed

4.Wat N, Wong RL, Wong IY. Associations between diabetic retinopathy and systemic risk factors. Hong Kong Med. J. 2016;22( 6):589-99. 
Crossref   Pubmed

5.Bazzazi N., Akbarzadeh S., Yavarikia M., Poorolajal J., Fouladi D.F. High myopia and diabetic retinopathy: A Contralateral Eye Study in Diabetic Patients With High Myopic Anisometropia. Retina. 2017 Jul; 37(7):1270 -1276. doi: 10.1097/IAE.0000000000001335.
Crossref   Pubmed

6.Bobr T. [Features of the course of diabetic retinopathy depending on the magnitude of the anterior-posterior axis of the eye]. Oftalmologiia. Vostochnaia Evropa. 2017;7(2):152-6. Russian.  

7.Moss SE, Klein R, Klein BE. Ocular factors in the incidence and progression of diabetic retinopathy. Ophthalmology. 1994;101(1):77-83. 
Crossref   Pubmed

8.Sultanov MI, Gadzhiev RV. [Features of the course of diabetic retinopathy in myopia]. Vestn Oftalmol. 1990;106(1):49-51. Russian. 

9.Man RE, Sasongko MB, Sanmugasundram S, Nicolaou T, Jing X, Wang JJ, Wong TY, Lamoureux EL. Longer axial length is protective of diabetic retinopathy and macular edema. Ophthalmology. 2012:119( 9):1754-9. doi: 10.1016/j.ophtha.2012.03.021. Epub 2012 May 23.
Crossref   Pubmed

10.Man RE, Sasongko MB, Wang JJ, Lamoureux EL. Association between myopia and diabetic retinopathy: a review of observational findings and potential mechanisms. Clin. Exp. Ophthalmol. 2013 Apr; 41(3):293-301. doi: 10.1111/j.1442-9071.2012.02872.x. Epub 2012 Oct 29.
Crossref   Pubmed

11.Mikheytseva IN, Mohammad Abdulhadi, Putienko AA, Kovalchuk AG, Kolomiichuk SG, Siroshtanenko TI. Modelling form deprivation myopia in experiment. Journal of Ophthalmology (Ukraine). 2018;2:50-5.

12.Beuerman RW, Maw SS, Tan DT et al. Myopia: animal models to clinical trials. Singapore World Scientific; 2010. 390 p.

13.Mohammad Abdulhadi, Mikheitseva IN, Putienko AA et al. [Rationale for a use of a form deprivation myipia model in studying pathogenesis and treatment of a disease].[Proceedings of scientific and practical conference of ophthalmologists of Chernyvtsi, Ivano-Frankivsk, Ternopil, Khmelnytskyi regions of Ukraine. 20-21 September 2017. Chernyvtsi]. Odessa, Chernyvtsi;2017:60-1. Russian.  

14.Bolshunov AV, Kulieva IA. [Characterisics of the clinical course of diabetic retinopathy in myopia]. Vestn Oftalmol. 1998;6:54-6. Russian.