https://doi.org/10.31288/oftalmolzh201367378

The state of retinal and vascular membranes of the rabbit eye with diabetes mellitus modeled by dithisone: report 6. Morphological comparison of the pigment epithelium of the retina in dithisone and streptosotocyne diabetes

Maltsev EV, Zborovska OV, Dorokhova OF, Chernyaeva SN

SI «The Filatov Institute of Eye Diseases and Tissue Therapy of NAMS of Ukraine»

Odessa, Ukraine

Introduction. The report 6 continues a series of publications on the state of retinal and vascular membranes of the animal eye with modeled diabetes mellitus (DM). 

Purpose. To characterize the state of the pigment epithelium of the retina (PER) completing the information published in the previous reports on morphological and metabolic shifts talking place in these eye structures in DM. Materials and methods. The investigation was made in Chinchilla rabbits, Wistar rats and CBA/C57B1xK/F1 mice that were induced DM. There were used up to 16 objects of each kind of animals. DM was modeled with the help of streptozotocyne in rats and mice, and dithisone was used in rabbits. There was made a histological comparison of PER state of the animal eyes.

Results. In advanced DM in rabbits there were easily seen deep changes both of the whole layer of PER and cells composing it in 16—17 weeks after the beginning of the experiment, thus supplementing the phenomena of the neurodegeneration of the retinal sensor part. As to the PER state in two other models of DM (in rats and mice) in both cases this structure of the retinas does not have such marked changes to be noted in routine examination staining of the preparations, for example, by hematoxylin-eosine. The same was noted in the neurodegeneration of the retinal sensor part of these animals.

Conclusion. Morphological changes of PER are clearly marked in dithisone model of DM in rabbits in contrast to stroptozotocyne DM of rats and mice.

Key words. Diabetic retinopathy, models of diabetes mellitus, structural changes of the retina, pigment epithelium of the retina

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