https://doi.org/10.31288/oftalmolzh201419397

Electronic — microscopic study of the ultrastructure of the chorioretinal complex of rats in dynamics after introduction of the increased dose of methanol

N. E. Dumbrova, N. I. Molchanjuk

SI «The Filatov Institute of Eye Diseases and Tissue Therapy of NAMS of Ukraine»

Key words: chorioretinal complex, ultrastructure, choriocapillaries, pigment epithelium of the retina, photore-ceptor cells

Introduction. A wide use of methanol and its availability increase probability of casual or its chronic influence on the human organism. Therefore, it is important to study its mechanisms for the person as well as other alive creatures. 

Purpose of the investigation. To study the influence of methanol on ultrastructure of the chorioretinal complex (CRC): (choriocapillaries (CC) — pigment epithelium of the retina (PER) — photoreceptor cells (PC) of the retina) of white rats. 

Material and methods. Electronic- microscopic changes in the elements of CRC of white rats in dynamics (1—14 day) after unitary intraabdominal introduction of methanol in the dose of 2.5 g/kg of body weight were investigated. 

Results and discussion. It is shown that the pathological process has a wavy course: on the 1st day the basic ultrastructural changes were revealed in CC and PER. In PER they consisted in alteration of mitochondria and canaliculi of the smooth endoplasmic net, in focal destruction of the plasmatic membranes, especially of the apical areas of cells. In PER cells signs of the endocellular compensatory-regenerative processes were revealed to the greatest degree on the 3rd day. However, damages of the structures were aggravated on the 7th — 14th day. 

Conclusions. Toxic effect of methanol in the dose of 2.5 g/kg of the body weight on CRC structures is manifested for a long time. Cells of PER react to the applied influence to the greatest degree

 

 

 

References

1.Bitenskii VS. The role of alcohol and drug abuse in the demographic crisis in Ukraine. Zhurnal AMN Ukrainy. 2007;13(3):543- 50.

2.Dolmatova LS, Romashina VV. Features of changes in the activity of antioxidant enzymes in different types of white blood cells in patients with chronic alcoholism. Pa-tologicheskaya fisiologiya I eksperimentalnaya terapiya. 2003;2:17-9.

3.Dumbrova Nye, Molchanyuk NN. Ultrastructural changes of chorioretinal complex elements of rat eyes after the action of methyl alcohol. Oftalmol Zh. 2009; 5: 54-7.
Crossref

4.Serov VV, Zabrodskii PF, Kirichuk VF. Effect of acute methanol poisoning on lipid peroxidation and concentration in blood corticosterone. Vestnik novykh med. Tehknologii. 2007;XIV(1):81-6.

5.Tsimbalyuk VI, Nosov AT, Chebatoreva LL, Vasyuta VA. Electrophysiological and morphological exponents,  the state of the visual analyzer in the dynamics of the Trophin application in the methanol intoxication. Ukrainskii neirokhirurgicheskii zhurnal. 2004; 3: 97- 102.

6.Eells JT, Salzman MM, Trusk TC. Inhibition of retinal mitochondrial function in methanol intoxication. Toxicologist.1995; 15: 21-3.

7.Martin-Amat G, McMartin KE, Hayreh SS, Tephly TR. Methanol poisoning: Ocular toxicity produced by formate. Toxicol Appl Pharmacol. 1978; 45: 201- 8.
Crossref

8.Treichel JL, Murray T G, Burton TC at all. Retinal toxicity in methanol poisoning. Retina. 2004;24:309- 12.
Crossref   Pubmed

9.Rajamani R, Muthuvel A, Senthilvelan M, Sheeladevi R. Oxidative stress induced by methotrexate alone and in the presence of methanol discrete regions of the rodent brain, retina and optic nerve. Toxicol. Lett. 2006; 12(5):12-5.