https://doi.org/10.31288/oftalmolzh201455360

Effect of sodium hyaluronate on pathochemical indicators in the tissues of the anterior eye after a preservative benzalkonium chloride application

V. Senishin

Lvov Regional Hospital; (Ukraine)         

Introduction. Importance of work is determined by clarification of the sodium hyaluronate effect on the pathochemical anterior eye tissue changes of animals treated with the instillation of preservative benzalkonium chloride (BAC).

Purpose. To study the effect of hyaluronate on the pathochemical indicators in the tissues of the anterior eye after application of the preservative benzalkonium chloride.

Methods. 22 rabbits were used for the experiment. All laboratory animals were divided into 2 groups: Group 1 — a control group (12 animals), the animals received the instillation of BAC, Group 2 — an experimental one (10 animals), the animals received the instillation of BAC and sodium hyaluronate. The activity of cytoplasmic, mitochondrial and lysosomal enzymes as well as reduced and oxidized glutathione were determined in the extracts obtained from the cornea, conjunctiva homogenates and lacrimal fluid.

Results. It was possible to weaken the negative effect of preservative BAC eye drops on the metabolic processes in the tissues of the anterior eye with hyaluronate. En­zyme activity was reduced under the influence of BAC, and the use of sodium hy-aluronate instillation restored enzyme activity: lactate dehydrogenase — 24.9 %, glucose-6phosphate dehydrogenase — 22.1 %, malate dehydrogenase — 23.9 %,glutamate dehydrogenase - 23.5 %, succinate dehydrogenase - 20.5 %, cyto-chrome oxidase - 27.3 %. Conclusions. Sodium hyaluronate significantly prevents the breakdown of enzy- matic activity caused by the preservative BAC in the tissues of the anterior eye.

Key words: the anterior eye, benzalkonium chloride, sodium hyaluronate 

References

1.     Gaidamaka TB, Senishin VI. Influence of the benzalkonium chloride on the oxidative-restored enzymes in the tissues of the anterior section of the eye. Oftalmol Zh. 2013; 5: 62-7..
Crossref

2.     Gaidamaka TB. Influence of preservatives of eye drops on the on the redox potential of glutathione in the tissues of the anterior eye. Oftalmpol Zh. 2012;6:96- 100. Russian.

3.     Ivanova NV. Preservatives in the topic therapy of eye diseases: advantages and disadvantages. Oftalmologiia. Vostochnaia Evropa. 2013;1(16):2-7. Russian.

4.     Nasledov A. SPSS computer analysis of data in psychology and social sciences. Spb.: Piter; 2005. 416 p.

5.     New methods of biochemical analysis. Izd. Leningradsk-ogo univer. 1991. 395 p.

6.     Ammar DA, Noecker RJ, Kahook MY. Effects of benzalkonium chloride- preserved, polyquad-preserved, and sofZia-preserved topical glaucoma medications on human ocular epithelial cells. Adv. Ther. 2010;27:1-9. 
Crossref

7.     Ayaki M, Yaguchi S, Iwasawa A. Cytotoxicity of ophthalmic solution with and without preservatives to human corneal endothelial cells, epithelial cells and conjunctival epithelial cells. Clin. Exp. Ophthalmol. 2008;36:553- 9.
Crossref

8.     Barki WH, Tahir M. Effects of topical benzalkonium chloride on corneal epithelium. Biomedica. 2007;23:65- 70.

9.     Bergmeyer HV. Metoden der enzymatischen Analyse. Herausgegeben von H. U. Bergmeyer. Berlin. 1986; 2220 p.

10.  Hopes M, Broadway DC. Preservative- free Treatment in Glaucoma Is a Sensible and Realistic Aim for the Future. Europ. Ophthalmic. Review. 2010;4:23- 8.
Crossref

11.  Hughes EH, Pretorius M, Eleftheriadis H. Long- term recovery of the human corneal endothelium after toxic injury by benzalkonium chloride. Br. J. Ophthalmol.2007;91:1460-3.
Crossref

12.  Kahook MY, Noecker RJ. Comparison of corneal and conjunctival changes after dosing of travoprost preserved with sofZia, latanoprost with 0,02 % benzalkonium chloride, and preservative- free artifical tears. Cornea.2008;27:339-43.
Crossref

13. Kim JR, Oh TH, Kim HS. Effect of benzalkonium chloride on the ocular surface of the rabbit. Jpn. J. Ophthalmol.2011;55:283-93.
Crossref

14. Kozobolis VP, Detorakis ET, Maskaleris G. Corneal sensitivity changes following the instillation of latanoprost, bitamoprost, and travoprost eyedrops. Am. J. Ophthalmol.2005;139:742-3.
Crossref

15. Liang H, Baudouin C, Pauly A. Conjunctival and corneal reactions in rabbits following short- and repeated exposure to preservative- free tafluprost, commercially avail­able latanoprost and 0,02 % benzalkonium chloride. Br.J. Ophthalmol. 2008;92: 1275-82.
Crossref

16. Lin Z, Liu X, Zhou T. A mouse dry eye model induced by topical administration of benzalkonium chloride. Mol.Vis. 2011;17:257-64.

17.  McCarey B, Edelgauser H. In Vivo corneal epithelial permeability following treatment with prostaglandin ana­logues with or without benzalkonium chloride. J. Ocul. Pharm. Ther. 2007;23:445- 7.
Crossref

18.  Trocme S, Hwang L— J, Bean GW. The role of benzalko-nium chloride in the occurrence of punctate keratitis: a meta- analysis of randomized, controlled clinical trials. Ann. Pharmacother. 2010;44:1914-21.
Crossref

19.  Whitson JT, Cavanagh HD, Lakshman N. Assessment of corneal epithelial integrity after acute exposure to ocular hypotensive agents preserved with and without benzalko-nium chloride. Adv. Ther. 2006;23:663- 71.
Crossref

20.  Wilson FM. Adverse external ocular effects of topical oph­thalmic therapy: an epidemiologic, laboratory, and clinical study. Tr. Am. Ophth. Soc. 1983;19:854- 8.

21.  Xiong C, Chen D, Liu J. A rabbit dry eye model induced by topical medication of a preservative benzalkonium chlo­ride. Invest. Ophthalmol. Vis. Sci. 2008;49:1850-6.
Crossref