Oftalmol Zh.2015;1:103-109
Influence of the corneal biological covering with cryocorserved amniotic membrane on the peculiarities of the course of modeled bacterial keratitis
G. I. Drozhzhina1, E. V. Sereda1, T. B. Gaidamaka1, V. V. Vit1, V. A. Shabliy2, G. S. Lobyntseva2
1 SI «Filatov’s Institute of eye diseases and tissue therapy of NAMN of Ukraine», Odessa, Ukraine
2 The Institute of Cellular Therapy, Kiev, Ukraine
Introduction. The study is important due to insufficient investigation of peculiarities of anti-inflammatory effect of the amniotic membrane in treatment of bacterial keratites.
Objective. To investigate in experiment the influence of the corneal biological covering with the amniotic membrane cryoconserved by a new technology on the peculiarities of the course of modeled bacterial keratitis.
Material and methods. Transplantation of the amniotic membrane cryoconserved by the new technology was performed in 30 Chinchilla rabbits (30 eyes) weighing 2.5–3.0 kg on the developed model of bacterial keratitis of the moderate degree of severity.
Results. In one month all animals showed absence of the amniotic membrane and the corneal surface was epithelized. It was characterized by formation of nonintensive keratoleucoma with residual infiltration in 3 rabbits (3 eyes). There was no vascularization in all animals. The histological samples showed complete epithelization with restoratopn of the corneal stroma. Edema and lymphocyte-histocytic infiltration were absent. There were preserved invaginations at the level of basal membrane (corneal plug).
Conclusion. It was established on the model of bacterial keratitis that amniotic membrane transplantation, cryoconserved by the developed technology stimulated epithelization being a biological covering and contributes to elimination of edema and signs of inflammatory reaction of the cornea.
Key words. Bacterial keratitis, amniotic cryoconserved membrane, experiment
References
1.Kasparov AA, Trufanov SV. Using preserved amniotic membrane for reconstruction of anterior segment surface. Vestn Oftalmol. 2003;3:45–7. In Russian.
2.Patent of Ukraine № 49759 «Activity of bank for storing cryopreserved biological objects». Bul. № 11. 15.11.2004. Lobyntseva G. S. Et al.
3.Patent of Ukraine № 87119 «A method of modeling bacterial keratitis of moderate severity». Bul № 2. 27.01.2014. Drozhzhina GI, Vansovich EV, Gaidamaka TB.
4.Trufanov SV. Using preserved amniotic membrane in human reconstructive eye surgery: Author’s thesis for Cand. Of Med. Science. 14.00.08 Eye Diseases. M., 2004. 24 p.
5.Fedorova EA. Application of lyophilized amniotic membrane in the treatment of inflammatory diseases of the cornea: Author’s thesis for Cand. Of Med. Science. 14.00.08 Eye Diseases. M., 2004. 24 p.
6.De Rotth A. Plastic repair of conjunctival defects with fetal membrane. Arch. Ophthalmol. 1940;23:522–5.
7.Gicquel JJ, Bejjani RA, Ellies P et al. Amniotic membrane transplantation in severe bacterial keratitis. Cornea. 2007;26:27–33.
8.Gris O, Del Campo Z, Wolley-Dod C et al. Amniotic membrane implantation as a therapeutic contact lens for the treatment of epithelial disorders. Cornea. 2002;21:22–7.
9.Hao Y, Ma DH, Hwang DG et al. Identification of antiangiogenic and antiinflammatory proteins in human amniotic membrane. Cornea. 2000;19:348–52.
10.Irina S Barequet, Zohar Habot-Wilner, Nathan Keller et al. Effect of Amniotic Membrane Transplantation on the Healing of Bacterial Keratitis. IOVS. 2007;23:124–30
11.Khokhar S, Natung T, Sony P et al. Amniotic membrane transplantation in refractory neurotrophic corneal ulcers: a randomized, controlled clinical trial. Cornea. 2005;24:654–60.
12.Kim JC, Tseng SC. Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas. Cornea. 1995;14:473–84.
13.Kim JS, Kim JC, Hahn TW, Park WC. Amniotic membrane transplantation in infectious corneal ulcer. Cornea.2001;20:720–6.
14.Kjaergaard N, Hein M, Hyttel L, et al. Antibacterial properties of human amnion and chorion in vitro. Eur J Obstet Gynecol Reprod Biol.2001;94:224–9.
15.Kruse FE, Rohrschneider K, Volcker HE. Multilayer amniotic membrane transplantation for reconstruction of deep corneal ulcers.Ophthalmology. 1999;106:1504–10.
16.Leibowitz HM. Bacterial keratitis. Leibowitz HM eds. Corneal Disorders: Clinical Diagnosis and Management. 1984.353. WB Saunders Philadelphia.
17.Meller D. Amniotic Membrane Transplantation in the Human Eye. Dtsch. Arztebl. Int. 2011;108(14):243–8.
18.Nordlund ML, Pepose JS. Corneal response to infection. Krachmer JH Mannis MJ Holland EJ eds. Cornea, Fundamentals, Diagnosis and Management. 2005;95–114. Elsevier Mosby Philadelphia.
19.Prabhasawat P, Tesavibul N, Komolsuradej W. Single and multilayer amniotic membrane transplantation for persistent corneal epithelial defect with and without stromal thinning and perforation. Br. J. Ophthalmol. 2001;85:1455–63.
20.Seitz B. Amniotic membrane transplantation. An indispensable therapy option for persistent corneal epithelial defects. Ophthalmologe. 2007;104:1075–9.
21.Solomon A, Meller D, Prabhasawat P, John T, Espana EM, Steuhl KP, Tseng SC. Amniotic membrane grafts for nontraumatic corneal perforations, descemetoceles, and deep ulcers. Ophthalmology. 2002;109(4):694–703.
22.Sorsby A, Symons HM. Amniotic membrane grafts in caustic burns of the eye: (Burns of the second degree). Br. J. Ophthalmol. 1946;30:337–45.
23.Talmi WP, Sigler L, Inge E et al. Antibacterial properties of human amniotic membranes. Placenta. 1991;12:285–8.
24.Thatte S. Amniotic membrane transplantation: An option for ocular surface disorders.Oman. J. Ophthalmol. 2011;4(2):67–72.
25.Tseng S, Prabhasawat P, Lee S. Amniotic membrane transplantation for conjunctival surface reconstruction. Am. J. Ophthalmol. 1997;124:765–74.
26.Tseng SC, Li DQ, Ma X. Suppression of transforming growth factor–beta isoforms, TGF–beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix. //J. Cell Physiol. 1999;179:325–35.