http://doi.org/10.31288/oftalmolzh201865964

Received: 07 September  2018; Published on-line: 31 December 2018

Efficacy of combined liposomal quercetin and yellow sweet clover aqueous extract in moderate blunt trauma to the eye in rabbits 

O.P. Sotnikova, Dr. Med. Sc., Prof.; N.O. Chudniavtseva, Dr. Med. Sc.;  G.S. Fesiunova, Cand. Biol. Sc.; Yu. M. Rodina, Cand. Med. Sc.; T.D.Lotosh, Cand. Biol. Sc.; G.B.Abramova, a researcher; G.M.Tsybuliak, a junior researcher 

Filatov Institute of Eye Diseases and Tissue Therapy of NAMS of Ukraine; Odessa (Ukraine)

E-mail: filatovscience@ukr.net

TO CITE THIS ARTICLE: Sotnikova OP, Chudniavtseva NO, Fesiunova GS, Rodina YuM, Lotosh TD, Abramova GB, Tsybuliak GM. Efficacy of combined liposomal quercetin and yellow sweet clover aqueous extract in moderate blunt trauma to the eye in rabbits. J.ophthalmol.(Ukraine).2018;6:59-64. http://doi.org/10.31288/oftalmolzh201865964  

 

Introduction. Despite the numerous experimental and clinical investigations, the development of new rational treatments for blunt trauma to the eye remains a topical issue.

Purpose. To study the efficacy of a combined use of liposomal Lipoflavon instillations and periocular injections of a yellow sweet clover aqueous extract for moderate blunt trauma to the eye in experiment. 

Material and Methods. Moderate blunt trauma (2 to 3 Nm) was accomplished transpalpebrally using a special device in 15 eyes of 30 rabbits. The animals were divided equally into two Study groups and Control Group: Study group 1, instillations of Lipoflavon and subconjunctival injections of a yellow sweet clover aqueous extract; Group 2, instillations of Lipoflavon and parabulbar injections of a yellow sweet clover aqueous extract; Control Group, untreated simulated pathology as controls. A degree of inflammatory response of the eye, a condition of the cornea, and hemorrhages into the anterior chamber were assessed using a scoring system which had been improved by us. Coagulogram characteristics including thrombin time (TT), prothrombin time (PTT), activated partial thromboplastin time (APTT), and fibrinogen were determined.

Results. Lipoflavon instillations and periocular injections of a yellow sweet clover aqueous extract for treating the eyes after moderate blunt were proved to have a pronounced therapeutic effect as compared to control, based on the clinical signs of the total post-traumatic response of the eye. The total response of the eye to blunt trauma was slightly decreased in the group receiving parabulbar injections of a yellow sweet clover aqueous extract as compared to the group receiving subconjunctival injections at Days 3 and 7 of the experiment. A combined use of Lipoflavon eye drops and periocular yellow sweet clover injections in treating the eyes after moderate blunt trauma results in changes in coagulation hemostasis indices towards hypocoagulation, which is evidenced by an increase in PTT averagely by 43% and a decrease of the fibrinogen level averagely by 25%.

Conclusions. Lipoflavon instillations in combination with periocular yellow sweet clover injections for experimental blunt trauma of moderate severity have a pronounced therapeutic effect comparing to untreated controls and result in changes in coagulation hemostasis indices towards hypocoagulation. 

Keywords:  experimental blunt trauma, Lipoflavon, yellow sweet clover aqueous extract, therapeutic efficacy

 

References

1.Verigo EN, Gundorova RA, Susaykova MS, Kudinova NG. [Specifics of Urgent Care of Victims of Emergency Situations with Ophthalmic Trauma]. Meditsina katastrof. 2009;4:15-18. Russian. 
Crossref

2.Krasnovid TA. [Successes and failures in rendering urgent medical aid for ocular trauma in Ukraine]. Problems of ecological and medical genetics and clinical immunology: a collection of papers]. Kyiv, Lugansk; 2001. 4(106):15-21. Russian. 

3.Blagun NV, Jia Ming. [Specialities of a clinical course, diagnostics and treatment of concussion hyphemas]. Ukrainskii naukovo-medychnyi molodizhnyi zhurnal. Spec. issue. 2011;1:13-14. Ukrainian.    

4.Verigo EN, Kuznetsova IA, Romanova IYu. [Conservative therapy and rehabilitation of patients with ocular injuries]. Vestn Oftalmol. 2002;118(2):34-7. Russian. 

5.Pasyechnikova NV, Gorshkova RA. [Clinical and biochemical rationale for using a Lipoflavon drug in patients with age-related cataract after cataract extraction and implantation of peroxidation products]. Ukrainskii med. almanakh.  2006;9(1):2014-18. Russian. 

6.Sotnikova OP, Fesiunova GS, Abramova GS, Lotosh TD, Sokolova BN, Saldan VI, Ivanov VI. [A useful model patent 3544, Ukraine, MPK 7А61К35/78. A technique for producing aqueous extract of yellow sweet clover]. No 20040504004; Appl. 26 May 2004; Publ. 15 November 2004; Bul. No11. Ukrainian. 

7.Sotnikova OP, Fesiunova GS, Lotosh TD. [Experimental studies on anticoagulative фтв fibrinolytic properties of yellow sweet clover aqueous extract]. Odeskii medychnyi zhurnal. 2005;3(89):39-42. Ukrainian.  

8.Fesiunova GS. [Effect of yellow sweet clover extract on the course of experimental hyphema]. Oftalmol Zh. 2007;3:55-60. Russian.

9.Rodina YuM, Chudniavtseva NO. [A useful model patent 64593 А Ukraine, 7 А61В3/18. A device for modeling blunt ocular trauma of various severity]. No 2003076482; Appl. 11 July 2003; Publ. 16 February 2004, Bul. No 2.

10.Logai IM, Leus NF, Georgiev DD et al. [Comparative study on the efficacy of anti-inflammatory agents on a model of post-operative inflammation]. Oftalmol Zh. 2001;3:85-92. Russian. 

11.Stanton A. Glantz. [Primer of biostatistics. Translated from English. Danilov YuA, the Translator]. M.:Praktika;1999:455. Russian.